紫椴花多糖的超声提取最佳条件和含量测定

设置7个萃取温度（25，35，45，55，65，75，85。C）和萃取时间（30，40，50，60，70，80，90min）来选择超声提取紫椴花中多糖的最佳提取温度和提取时间。利用分光光度法对紫椴花中的多糖含量进行了分离和测定。结果表明：最佳提取条件为提取温度为75℃，提取时间为52min。经蒽酮—硫酸显色，于580nm处测定，其多糖的含量为9．74％，RSD=0．47％（n=3）。此测定方法简便，样品溶液在4h内显色稳定，重现性较好，平均回收率为99．48％±1．01％，RSD=0．112％（n=3）。

Optimal ultrasonic extraction condition and determination of polysaccharides in <Emphasis Type="Italic">Tilia amurensis</Emphasis> flowers

Seven extracting temperatures (25, 35, 45, 55, 65, 75, 85°C) and extracting time (30, 40, 50, 60, 70, 80, 90 min) were designed for selecting the optimal extracting time and extracting temperature for ultrasonic extraction of T. amurensis flowers. Polysaccharides from T. amurensis flowers were isolated and determined by spectrophotometry. Results show that the optimal ultrasonic temperature was 75°C and extracting time 52 min. The content of polysaccharides in T. amurensis flowers measured by anthrone—H₂SO₄ colorimetry under 580 nm, was 9.74% with 0.47% of relative standard deviation (RSD, n=3). This study demonstrated that ultrasonic extraction method was simple, and the color of the treated samples was stable in 4 h. The average recovery value for the polysaccharides measured was 99.48%±1.01%, with 0.112% of RSD (n=3).