| 转基因水稻中转人乳铁蛋白的抗氧化及免疫作用 |
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| 引用本文： | 秦毅,吕国英,张作法. 转基因水稻中转人乳铁蛋白的抗氧化及免疫作用[J]. 浙江农业学报, 2018, 30(1): 1. DOI: 10.3969/j.issn.1004-1524.2018.01.01 |
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| 作者姓名： | 秦毅 吕国英 张作法 |
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| 作者单位： | 1.浙江大学 技术转移中心,浙江 杭州 310058; 2.浙江省农业科学院 园艺研究所,浙江 杭州 310021 |
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| 基金项目： | 浙江大学基本科研业务费专项资金项目(2015XZZX002-03) |
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| 摘 要： | 以转人乳铁蛋白为研究对象,研究其抗氧化和免疫活性。采用DPPH法、ABTS法和羟基自由基清除能力的方法来研究转人乳铁蛋白(rhLF)的抗氧化功能,通过小鼠巨噬细胞RAW264.7的细胞增殖和吞噬活性的检测来测定转人乳铁蛋白的免疫活性。结果表明,转人乳铁蛋白对DPPH、ABTS和羟基自由基具有一定的清除能力,并能促进免疫细胞的增殖和增强免疫细胞的吞噬活性。转人乳铁蛋白具有较好的免疫活性和抗氧化活性,是开发相关保健食品的重要资源。
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| 关 键 词： | 转人乳铁蛋白 抗氧化 免疫作用 |
| 收稿时间： | 2017-06-29 |
Studies on immunomodulatory and antioxidant activities in vitro of recombinant human lactoferrin from transgenic rice |
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| QIN Yi,LYU Guoying,ZHANG Zuofa. Studies on immunomodulatory and antioxidant activities in vitro of recombinant human lactoferrin from transgenic rice[J]. Acta Agriculturae Zhejiangensis, 2018, 30(1): 1. DOI: 10.3969/j.issn.1004-1524.2018.01.01 |
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| Authors: | QIN Yi LYU Guoying ZHANG Zuofa |
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| Affiliation: | 1. Technology Transfer Center, Zhejiang University, Hangzhou 310058, China;
2. Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China |
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| Abstract: | In the present study, the immunomodulatory and antioxidant activities of the recombinant human lactoferrin (rhLF) were studied. The antioxidant activities of rhLF were investigated by DPPH, ABTS and hydroxyl radical scavenging activities and the immunomodulatory activities were determined by the detection of cell proliferation and phagocytic activity of RAW264.7. It turns out that rhLF exhibited scavenging activity against DPPH, ABTS and OH· radical as well as immunomodulatory activities. rhLF possessed excellent antioxidant and immunomodulatory activities, and was the important resource of the exploitation of health-care food. |
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| Keywords: | recombinant human lactoferrin antioxidant activity immunomodulatory activity |
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