免疫诱导剂处理的黑李叶片蛋白质组学分析

以黑李品种黑宝石李为试材,用免疫诱导剂保康灵1号处理黑李叶片,测定叶片生理生化指标,并进行蛋白质组学分析。结果表明,保康灵1号处理后黑李叶片明显变大,叶面积增加,叶片变厚,叶绿素含量和多酚氧化酶活性增加。叶片横切面显示,诱导剂处理后叶片内栅栏组织、海绵组织和下表皮明显增厚。说明诱导剂处理后黑李叶片内栅栏组织和海绵组织增厚可能是致叶片变厚和叶绿素含量增加的原因。蛋白质组学分析结果表明,共筛选出157个差异蛋白,其中上调蛋白75个(上调1.5倍且P<0.05),下调蛋白82个(下调1.5倍且P<0.05)。GO分类和代谢途径分析结果发现差异上调蛋白主要分布在叶绿体、叶绿体膜和叶绿体基质中,而差异下调蛋白主要分布在叶绿体基质、叶绿体被膜和光系统Ⅰ,共同参与光合作用。综上所述,免疫诱导剂保康灵1号可能通过调节黑李叶片光合作用相关蛋白表达,促进叶片生长发育。

Proteomic analysis of black plum leaf treated with immunity inducer

The plum variety Friar plum was used as test material. Black plum leaves were treated with immunity inducer Baokangling No.1. Proteomic analysis and determination of physiological and biochemical indexes of treated leaves were carried out. The results showed that area, thickness, chlorophyll content and PPO activities of black plum leaf treated with immunity inducer Baokangling No.1 all increased. The cross section of blade showed that the palisade tissue, spongy tissue and lower epidermis were obviously thickened after treatment with immunity inducer, which was probably responsible for leaf thickening and increased chlorophyll content. Proteomic analysis showed that a total of 157 differentially expressed proteins were screened, including 75 up-regulated proteins (up to 1.5 fold and P<0.05) and 82 down-regulated proteins (down 1.5 fold and P<0.05).GO classification and KEGG pathway analysis showed that differentially up-regulated protein (or genes) were enriched and assigned to the chloroplast, chloroplast membrane and chloroplast matrix function, whereas differentially down regulated proteins (or genes) were enriched and assigned to chloroplast matrix, the chloroplast membrane and photosystem Ⅰ. Both of them were involved in photosynthesis. Taken together, the immunity inducer Baokangling No.1 may promote the growth of black plum leaves via regulating the expression of proteins related to photosynthesis.