3种三唑类杀菌剂对斑马鱼的毒性研究

探究戊唑醇、丙环唑、苯醚甲环唑3种三唑类杀菌剂对斑马鱼的急性毒性、两两等毒性和等浓度配比时对斑马鱼的联合毒性。同时,通过6 d胚胎发育试验,研究3种三唑类杀菌剂对斑马鱼胚胎的发育毒性。研究显示,戊唑醇、丙环唑、苯醚甲环唑3种三唑类杀菌剂对斑马鱼胚胎、仔鱼和成鱼的急性毒性为中毒,苯醚甲环唑和丙环唑在等毒性和等浓度配比下对斑马鱼胚胎和仔鱼阶段为协同作用,其余组合对斑马鱼胚胎和仔鱼阶段为拮抗作用。发育毒性试验结果表示,3种三唑类杀菌剂对早期生命阶段毒性更强,可诱导斑马鱼胚胎、仔鱼出现一系列不良症状,包括孵化率下降、心率异常、生长抑制等。

Study on toxicity of three kinds of triazole fungicides on zebrafish (Danio rerio)

The acute toxicity of three kinds of triazole fungicides (tebuconazole, propiconazole, difenoconazole) to zebrafish was explored, and the joint toxicity of two drugs under conditions of equivalent toxicity and equivalent concentration was explored. At the same time, a six-day embryonic exposure was also carried out to investigate the developmental effect of three triazole fungicides. The study showed that the acute toxicity of the three kinds of triazole fungicides (tebuconazole, propiconazole, difenoconazole) to embryo, larvae and adult fish of zebrafish were medium; difenoconazole and propiconazole had synergistic effects on the embryo and fish larvae of zebrafish under conditions of equivalent toxicity and equivalent concentration; the rest combination of two drugs have antagonistic effects on the embryo and larvae of zebrafish. The results of developmental toxicity test indicated that the three triazole fungicides were more toxic to zebrafish at an early life stage and could induce a series of adverse symptoms in zebrafish embryos and larvae, including decreased hatching rate, abnormal heart rate and growth inhibition, etc.