复方中药制剂对SD大鼠胸腺组织结构及Caspase-3蛋白分布的影响

为研究由炒白术、山楂、黄芪、板蓝根、茯苓、陈皮、甘草、山药制成的复方中药制剂对SD大鼠胸腺组织结构及其Caspase-3蛋白分布的影响。选择60只SPF级断奶SD大鼠,随机分为对照组、1.5 mL·kg^-1组、3.0 mL·kg^-1组和6.0 mL·kg^-1组,每组15只,预饲1周后按每千克体质量分别定时灌胃3.0 mL蒸馏水、1.5 mL、3.0 mL和6.0 mL药液(每mL含1 g生药)。至90 d,各处理组SD大鼠禁食禁水3 h,称量、麻醉、处死后取完整的胸腺称量,立即投入4%的多聚甲醛磷酸缓冲液固定72 h,制作石蜡组织切片,经HE、网状纤维和Caspase-3免疫组化染色,显微观察,Image-pro PLUS 6.0软件分析并摄影。结果表明,按每kg体质量灌胃本复方中药制剂3.0 mL可显著增加胸腺皮质区且胸腺的生长指数显著高于对照组(P<0.05)。网状纤维主要分布于SD大鼠胸腺髓质部,3.0 mL·kg^-1剂量组网状纤维含量较对照组显著减少。各实验组均不同程度抑制Caspase-3蛋白的出现,其中以3.0 mL·kg^-1剂量组最为显著。说明灌胃3.0 mL·kg^-1复方中药制剂可改善SD大鼠胸腺组织结构,延缓其结构和功能退化。

Effects of compound traditional Chinese medicine preparation on thymus tissue structure and caspase-3 protein distribution of SD rats

To study the effects of compound traditional Chinese medicine preparation on the thymus tissue structure and Caspase-3 protein distribution of SD rats, which consist of Atractylodes macrocephala, Crataegus pinnatifida Bunge, Astragalus membranaceus (Fisch.) Bunge, Isatis tinctoria, Poria cocos Wolf, Pericarpium citri Reticulatae, Radix glycyrrhizae and Dioscoreae rhizoma. 60 SD rats (SPF animal), were randomly divided into control treatment group, compound traditional Chinese medicine soup 1.5, 3.0 and 6.0 mL·kg^-1 treatment groups, with 15 rats in each. After pre-feeding for one week, 3.0 mL distilled water, 1.5 mL, 3.0 mL and 6.0 mL soup according to mass per kilogram were applied by timing gavage, respectively. On the 90^th day, each treatment group rats were fasted and banned water for 3 h, then weighed, anaesthetized and put to death. The full thymus were taken and weighed, and were immediately immersed into the 4% polyformaldehyde phosphate buffer and fixed for 72 h, for making paraffin section, by HE, reticular fiber and caspase-3 immunohistochemical dyeing, microscopic observation, Image-pro PLUS 6.0 software analysis and photography. The results showed that 3.0 mL compound traditional Chinese medicine preparation treatment could increase obviously cortex of thymus according to the mass per kilogram, and the growth index of thymus gland was significantly higher than that of the control group (P<0.05). The reticular fibers were mainly distributed in the thymus medulla of SD rats, and the content of reticular fiber in 3.0 mL·kg^-1 dose group was significantly lower than that of the control group. Each test group could inhibit the appearance of caspase-3 protein in different degrees, the inhibition effect of the 3 mL·kg^-1 dose group was the most significant. It indicated that 3 mL·kg^-1 compound Chinese medicine preparation treatment could improve the thymus tissue structure of SD rats, delay its structure and function degradation.