闽西地区猪流行性腹泻病毒M基因遗传进化及分子结构特征分析

为了解2015—2017年闽西地区猪流行性腹泻病毒(PEDV)遗传进化规律,本研究收集了闽西地区12份PEDV阳性样品,对其M基因进行RT-PCR扩增,克隆至pMD18-T载体测序,并与国内外已知参考毒株序列进行比对及遗传进化分析。结果表明,12株闽西毒株M基因氨基酸序列同源性为100.0%;遗传进化分析表明,12株闽西毒株与经典毒株CV777、2010年前中国分离毒株LCZ、以及韩国毒株SM98、virulent DR13的亲缘性较远,而与2010年后国内分离毒株以及2013年美国分离毒株亲缘关系最近,说明闽西PEDV毒株属于目前国内外流行毒株。分子结构特征分析表明,PEDV M基因具备高度保守的特性,可以作为设计疫苗的候选基因。

Evolutionary and molecular characteristics of M genes of porcine epidemic diarrhea viruses in western Fujian,China

To elucidate evolutionary characteristics of the memberane (M) genes of porcine epidemic diarrhea viruses in Fujian, China, 12 samples collected from Fujian province during 2015 to 2017 were screened by multiplex RT-PCR,M genes of PEDV viruses from positive samples were amplified by RT-PCR and cloned into pMD18-T vectors for sequencing respectively. The sequence data were done alignment and phylogenetic analysis with sequences from known reference strains. The M genes from 12 representative positive samples shared 100.0% identity at amino acid level. 12 representative positive samples are remote to classical strain CV777, SM98 and virulent DR13. 12 representative strains belong to the genotype variant strain. Molecular structure characteristics analysis showed that M gene is highly conservative and could be an optimal candidate protein for vaccine development.