鹅褪黑素受体Mel1c基因的组织表达特征

采用qRT-PCR等技术探究了Mel1c在鹅不同器官组织(心脏、肝脏、肾脏、肺脏、胰脏、脾脏、卵巢、大脑、胸肌及F1、F2、F3、F4、F5卵泡)中的表达分布。结果表明,Mel1c mRNA在鹅的心脏、肝脏、肾脏、肺脏、胰脏、脾脏、卵巢、大脑、胸肌及各级卵泡中均有表达;其表达量在各组织中存在差异,且以胰脏、脾脏、肾脏、卵巢中表达量较高,肺脏次之,心脏、胸肌、大脑水平相当,肝脏中最少。而在卵泡组织中,F4级卵泡中最高,其次为F5级卵泡,F1级卵泡中表达量最少。Mel1c在鹅各组织中普遍表达分布,进一步说明了褪黑素在机体中有着广泛的生物学作用,特别是对卵泡发育的影响。

Expression of Mel1c mRNA in various tissues of goose

This research investigated the expression of Mel1c in different tissues of goose (including heart, liver, kidney, lung, pancreas, spleen, ovary, brain, breast muscle and F1, F2, F3, F4, F5 stage follicles) using qRT-PCR. The results showed that Mel1c mRNA was expressed in the above mentioned tissues of goose, but the expression levels of Mel1c mRNA were different among various tissues. The expression levels of Mel1c mRNA in pancreas, spleen, kidney and ovary were higher than that in other tissues, followed by lung, the expressions in heart, breast muscle and brain were at a similar level, and the least was in liver. The expression levels of Mel1c mRNA were also different among various stages of follicles. The highest expression of Mel1c mRNA was found in F4 follicles, followed by F5 follicles, and the least was in F1 follicles. Thus, Mel1c was widely expressed in goose tissues, which further confirmed its involvement in the broad physiological functions of melatonin, especially the development of follicles in ovary.