感染鸭源小鹅瘟病毒QH-L01株樱桃谷雏鸭病理学研究

探究人工感染条件下,鸭源小鹅瘟病毒QH-L01株对樱桃谷雏鸭各器官组织病理学变化的影响,以期阐明QH-L01的病理发生规律及特征。实验组以每只0.2 mL(10^6.54 ELD₅₀)的剂量肌肉接种30只2日龄樱桃谷肉鸭鸭源小鹅瘟病毒,对照组的鸭接种等量的灭菌生理盐水,分别在1、3、6、9 d观察各组织器官的病理变化。感染后1 d,先出现肺点状出血,部分肺小叶扩张,十二指肠、回肠和盲肠等肠道组织绒毛上皮细胞坏死脱落。感染后3 d,脑、肾、胸腺、法氏囊出现病理变化,十二指肠、回肠和盲肠等肠道组织绒毛和黏膜层轻度坏死、脱落。感染后6 d,脾脏淋巴细胞增多,十二指肠、回肠和盲肠等肠道组织绒毛和黏膜层中度坏死、脱落。感染后9 d,各组织病变减轻或者恢复正常。整个感染期死亡率为20%。

Pathological study on duck-origin goose Parvovirus strain QH-L01 infection of Cherry Valley duck

In the present study, the histopathological changes of various organs of Cherry Valley ducklings were explored under the condition of artificial infection, to clarify the pathogenesis and characteristics of duck-origin goose Parvovirus strain QH-L01. Thirty Cherry Valley ducks in experimental group were intramuscularly inoculated with 0.2 mL of 10^6.54 ELD₅₀ duck-origin goose Parvovirus strain, and the ducks in the control group were intramuscularly inoculated with 0.2 mL of sterilized normal saline per duck, to observe the pathological changes of various tissues/organs in each infective stage at 1, 3, 6, 9 d, respectively. On the 1^st day after infection, the lungs appeared punctate hemorrhage, partial lobular expansion, and intestinal tissue villous epithelial cells necrosed. On the 3^rd day after infection, brain, kidney, thymus, bursa fabricius showed pathological changes, and villus of duodenum and ileum slight necrosed, mucosa layer of cecum appeared slight necrosis. On the 6^th day after infection, lymphocytes in spleen increased, moderate necrosis was detected in villus of duodenum, ileum and mucosa layer of cecum. On the 9^th day after infection, the lesions were reduced or returned to normal. The mortality rate was 20% during the whole infection period.