免疫胶体金法快速检测水产品中喹诺酮类药物

利用QH12杂交瘤细胞株生产抗体,研制高灵敏度的免疫胶体金试剂条,其对洛美沙星、氧氟沙星、诺氟沙星、培氟沙星的最低检出限分别为2.0、2.5、2.5、5.0 ng·mL^-1。通过样品预处理方法优化,建立免疫胶体金快速检测方法(GICT)。将其与HPLC法进行比较,并用LC-MS/MS法验证效果。结果表明,GICT灵敏度高于HPLC法,检测结果与LC-MS/MS法较一致,单个样品检测耗时25 min。综合比较分析,GICT宜作为水产品中喹诺酮类药物残留的快速筛查检测方法进行推广。

Rapid determination of quinolones in aquatic products by immune colloidal gold method

Antibodies were produced by using the hybridoma cell line QH12, and the highly sensitive immune colloidal gold reagent strip was thus prepared. The lowest detection limits of these stripes for lomefloxacin, ofloxacin, norfloxacin, pefloxacin were 2.0, 2.5, 2.5, 5.0 ng·mL^-1, respectively. The colloidal gold rapid test methods (GICT) for determination of quinolones residues in aquatic products were developed through optimization of sample pretreatment. Detection results of GICT were further compared with that of HPLC method, which was confirmed by LC-MS/MS method. It was shown that the sensitivity of GICT method was higher than that of HPLC. The test results of GICT and LC-MS/MS were in coincidence. The whole detection process of a single sample cost 25 minutes. Comprehensive comparative analysis showed that GICT method was suitable for rapid determination of quinolones in aquatic products.