猪囊虫病基因工程疫苗用于免疫治疗的研究

应用猪囊虫病基因工程疫苗分别对实验性猪囊虫病和自然感染的猪囊虫病进行了分组治疗，以探讨该疫苗的免疫治疗作用。１０头人工复制的囊虫病猪，随机分成３组。第１组４头猪，于感染于１个月注射该疫苗，连续３次，间隔１５天；第２组４头猪，于感染后２个月注射疫苗，同上连续３次；第３组２头猪，不注苗。第１组注射疫苗后１个月循环抗原滴度开始降低，２．０、２．５及３．５个月各有１头猪ＣＡ转阴，５个月ＯＤ值分别为０．１４、０．０９、０．１７、０．３８。第２组和第３组各猪血清ＯＤ值一直维持在１．０以上。感染后５个月剖检，第１组４头猪均未检出囊虫；第２组４头猪在咬肌、膈肌、腰肌、股内侧肌、肩胛外侧肌等部位均检测到囊虫，检测部位４０ｃｍ＾２面积发现囊虫３～７个，有部分虫体已钙化，胆汁卵化率为１５．４％；第３组各猪也在各检测部位检测到囊虫，４

Immunotherapy for Swine Cysticercosis with the Genetic Engineering Vaccine

In order to probe the effects of the immunotherapy for swine cysticercosis with the genetic engineering vaccine,the pigs infected experimentally or naturally with cysticercosis were treated with the vaccine. 10 pigs which were infected experimentally cysticercosis were randomly divided into three groups: l)four pigs,each was injected the genetic engineering vaccine against swine cysticercosis after one month of infection for three times at interval of fifteen days; 2)four pigs,each was injected the vaccine as above after two months of infection; 3)two pigs,each was injected nothing.The sera CA of all the infected pigs were positive,which were detected by sandwich ELISA,and their OD values were between 0.9 and 1.42 after one month of infection.In the first group,the OD values began to decrease in one month of the first injection of the vaccine,and one of the CAs was negative in 2.0,2.5and 3.5 months,respectively,no cysticerci was found.In the second group,cysticerci was found in the detected muscles of all the four pigs:masseter,septonles,psoas,vastus medialis and scapulae externus.The number of cysticerci hosted in the detected pork was 3 to 7 per 40 cm 2.Their bile hatching rates were 15.4%.In the third group,the cysticerci were detected in the two pigs,the number of cysticerci hosted in the detected pork was 6 to 9 per 40 cm 2,their bile hatching rates were 90.4%.By epidemic investigation detecting the sera's CA,we confirmed that 25 pigs were infected naturally with cysticercosis.They were divided into three groups as their OD values and were injected with the genetic engineering vaccine as above: 1)7 pigs,their OD values between 0.68 and 0.90; 2)8 pigs,their OD values between 0.91 and 1.10; 3)10 pigs,their OD values between 1.11 and 1.54.Six months later,no cysticerci was found in all the pigs of the first group,in 5 pigs of the second group and in 2 pigs of the third group,respectively.These results revealed that the genetic engineering vaccine could play important role in the therapy to swine cysticercosis,and have better the effect in earlier therapy.