| PtAP3不育结构转化毛白杨的研究 |
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| 引用本文： | 于来,安新民,曹冠琳,陈仲,张志毅.PtAP3不育结构转化毛白杨的研究[J].北京林业大学学报,2010,32(5):15-20. |
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| 作者姓名： | 于来 安新民 曹冠琳 陈仲 张志毅 |
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| 作者单位： | 北京林业大学林木育种国家工程实验室，林木花卉遗传育种教育部重点实验室，国家林业局树木花卉育种与生物工程重点开放实验室 |
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| 基金项目： | "948"国家林业局引进项目，"863"国家高技术研究发展计划项目 |
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| 摘 要： | 毛白杨是优良的绿化和用材树种,但是毛白杨雌株在蒴果成熟时有飞絮现象,给人们的生产和生活带来诸多不便。为获得不飞絮的不育毛白杨,将利用不育结构35S-PtAP3-IR-NOS对毛白杨进行转化,经PCR验证,共获得阳性转化植株12株,通过荧光定量检测转基因植株中外源基因AP3的表达量,发现不同株系间表达量一致,差异较小,相对表达量最高的株系为内参的4.96倍,最低的株系为内参的3.02倍。转基因植株中的上游基因LFY、PI的相对表达量与野生型对照相比下降明显,而下游基因SEP2、SEP3、SEP4的相对表达量与野生型对照比较没有太大变化。这些研究结果表明:转35S-PtAP3-IR-NOS不育结构的毛白杨阳性植株中AP3、LFY、PI基因的表达受到明显抑制,对毛白杨花发育将起到一定的抑制作用。本研究为利用转基因手段获得不育材料提供了依据。
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| 关 键 词： | PtAP3 毛白杨 遗传转化 抑制开花 |
| 收稿时间： | 1900-01-01 |
Genetic transformation of Populus tomentosa Cart. with sterility construct of PtAP3 |
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| YU Lai,AN Xin-min,CAO Guan-lin,CHEN Zhong,ZHANG Zhi-yi.Genetic transformation of Populus tomentosa Cart. with sterility construct of PtAP3[J].Journal of Beijing Forestry University,2010,32(5):15-20. |
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| Authors: | YU Lai AN Xin-min CAO Guan-lin CHEN Zhong ZHANG Zhi-yi |
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| Institution: | National Engineering Laboratory for Tree Breeding，Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration，Beijing Forestry University, 100083,P.R.China. |
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| Abstract: | National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration,Beijing Forestry University,100083,P.R.China.Populus tomentosa Carr.is a native species in section Leuce with fast-growing and high wood quality in northern China.However,its catkin pollution brings a lot of inconvenience to people's life and production activity... |
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| Keywords: | PtAP3 Populus tomentosa Carr genetic transformation suppressed flowering |
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