| 鳖病原细菌的分离鉴定及胞外产物的初步分析 |
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| 引用本文： | 张春嵋,王建生,邵碧英,吴祖建,林奇英,谢联辉.鳖病原细菌的分离鉴定及胞外产物的初步分析[J].福建农林大学学报(自然科学版),1999,28(1):90-95. |
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| 作者姓名： | 张春嵋 王建生 邵碧英 吴祖建 林奇英 谢联辉 |
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| 作者单位： | 福建农业大学植物病毒研究所,福州,350002 |
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| 摘 要： | 对来自福建光泽、宁德和福州的表现不同症状的病鳖进行细菌分离，所得菌株经形态观察及生化反应鉴定均为有运动力的气单胞菌，其中主要是嗜水气单胞菌（Aeromonashydrophila）．对气单胞菌进行了致病性和药物敏感性试验，对其液体培养物用（NH4）2SO4沉淀得到胞外产物，测得其对免血有溶解性，对小白鼠有致死也．SDS－PAGE电泳结果表明，胞外产物的差异具有菌株特异性．
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| 关 键 词： | 鳖 病原 嗜水气单胞菌 胞外产物 |
| 修稿时间： | 1998年7月6日 |
Isolation and identitication of the bacterial pathogens of soft-shelled turtles and preliminary analysis of their extracellular products |
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| Zhang Chunmei, Wang Jiansheng, Shao Biying, Wu Zujian, Lin Qiying, Xie Lianhui.Isolation and identitication of the bacterial pathogens of soft-shelled turtles and preliminary analysis of their extracellular products[J].Journal of Fujian Agricultural and Forestry University,1999,28(1):90-95. |
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| Authors: | Zhang Chunmei Wang Jiansheng Shao Biying Wu Zujian Lin Qiying Xie Lianhui |
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| Abstract: | Pathogenic bacteria were isolated from diseased soft-shelled turtles. The isolates were identified to be mobile aeromonases, mainly Aeramonas hydrophila, by morphological and biological characterization. The pathogenicity and sensitivity to antibiotics of the isolates were tested. Extracellular products were obtained by precipitation from the culture fluid using ammonium sulphate, and were proved to be with hemolysis and lethality for mice. SDS-PAGE showed that the differences between extracellular products were strain-specific. |
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| Keywords: | soft-shelled turtle pathogen Aeromonas hydrophila extracellular product |
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