Development of a sequence-specific PCR marker linked to the Ku gene which removes the vernalization requirement in narrow-leafed lupin |
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Authors: | J. G. Boersma B. J. Buirchell K. Sivasithamparam H. Yang |
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Affiliation: | Department of Agriculture and Food, Western Australia, 3 Baron-Hay Court, South Perth, WA 6151, Australia;;The University of Western Australia, 35 Stirling Highway, Nedlands, WA 6009, Australia;Corresponding author, E-mail: |
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Abstract: | Wild types of Lupinus angustifolius require vernalization to promote flowering. Modern domesticated cultivars carry the early-flowering gene Ku which removes this requirement. A microsatellite-anchored fragment length polymorphism marker was identified as co-segregating with the Ku gene in a recombinant inbred line (RIL) population derived from a domesticated × wild-type cross. DNA sequencing showed that the marker contained a 7 bp insertion/deletion polymorphism, as well as a single nucleotide polymorphism. A pair of sequence-specific primers was designed and successfully converted the size polymorphism into a simple polymerase chain reaction based co-dominant marker. This marker is closely linked to the Ku gene, as it co-segregates with the Ku phenotyping in a population consisting of 106 RILs. |
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Keywords: | Lupinus angustifolius early flowering simple sequence repeat–PCR marker |
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