玉米单倍体诱导、加倍技术及相关机理探讨

以生物诱导为基础的DH育种已发展成为玉米育种的核心关键技术,能够显著缩短自交系选育周期,加快育种进程。近年来,单倍体规模化诱导技术已经相当成熟,但批量加倍还是亟待解决的技术难题。文章综述玉米单倍体获得的技术途径、加倍措施、诱导率和加倍率QTL定位及相关基因的研究进展,对单倍体的不同加倍方法以及不同加倍药剂的加倍效果进行分析,通过元分析剖析诱导位点的遗传结构。

Analysis of Technique and Mechanism Relate to Induction and Doubling of Haploid in Maize

DH breeding based on biological induction has developed into a key technology of maize breeding, application of DH technology can significantly shorten breeding cycle, improve the breeding efficiency and accelerate the breeding process. In recent years, the large-scale induction technique of haploid has been quite mature, but batch doubling is still an urgent technical problem. In this paper, new research progress of haploid obtained approaches, doubling technical measures, genes or QTLs of induction rate and doubling rate were reviewed; the effectiveness of different doubling methods and different doubling reagents was also analyzed; furthermore, the genetic architecture of induction locus was dissected by Meta-QTL analysis, which would provide the reference to further research on haploid breeding technology.