EMS诱变玉米自交系种质创新应用

利用0.5 mg/L EMS诱变玉米自交系K22及B73花粉，构建2 206个株系不同遗传背景的玉米突变体库，从M₂和M₃后代筛选到包括株型、叶型、叶色、育性、抗逆、表皮形态建成等表型变异的一系列突变体。具有育种应用潜能的E16、E199和W594突变材料，可供用于选育矮秆、低穗位高、耐密植抗倒伏型新品种。对调控植物表皮形态建成的E88和sep-1（smooth epidermal pavement cell lobes-1）突变体进行表皮形态、发育模式鉴定及遗传学分析，构建了F_2：3分离群体。

Innovative Application of EMS Mutagenesis Germplasm of Maize Inbred Lines

A maize mutant library with 2 206 maize mutant libraries was constructed by mutagenesis of maize inbred line B73 and K22 pollen, which was treated with 0.5 mg/L ethyl methanesulfonate solution(EMS). A series of mutants with phenotypic variation including plant type, leaf type, leaf color, fertility, resistance, and epidermal morphogenesis, were screened from M₂ and M₃ progeny. E16, E199 and W594 mutants with potential breeding application were mainly described which could be used for breeding new varieties of dwarf, low-ear and high density and lodging resistance dense-tolerant. In addition, epidermal morphological and developmental pattern identification and genetic analysis were performed on E88 and sep-1(smooth epidermal pavement cell lobes-1) mutants regulating epidermal morphogenesis of plants. Subsequently, F_2:3 isolates population was constructed and candidate genes regulating epidermal morphogenesis will be clone by mapping.