连作对马铃薯酚酸类自毒物质及根际真菌群落的影响

为研究连作对马铃薯根际土壤中酚酸类自毒物质及真菌群落的影响,通过收集不同连作季(1季、3季、5季)马铃薯根际土壤,利用高效液相色谱法测定酚酸类物质及Illumina高通量测序技术测定真菌ITS序列,结果表明:(1)连作土壤中检测出丁香酸、香豆酸、对羟基苯甲酸、阿魏酸,连作增强后,pH不断下降,对羟基苯甲酸、阿魏酸含量不...

Effect of Continuous Cropping on Autotoxic Substances and Rhizosphere Fungi Communities in Potato

By collecting the potato rhizosphere soil from different continuous seasons,which includ 1-season (CK), 3-season, and 5-season periods.The HPLC method was used to determinate phenolic acids, the Illumina high-throughput sequencing technology was used to determinate ITS sequences, the effect of continuous cropping on potato autotoxins and rhizosphere fungi communities was studied for providing theoretical basis to eliminate the obstacles for potato continuous cropping.The results revealed that (1) The contents of 4 phenolic acids,including syringic acid, coumaric acid, p-hydroxybenzoic acid and ferulic acid, were detected in the continuous cropping soil.The contents of phydroxybenzoic acid and ferulic acid increased continuously while the pH declined constantly. The contents of syringic acid and coumaric acid were the highest in the 3-season continuous cropping soil. (2) The diversity and abundance of fungi in the 3-season and 5-season continuous cropping rhizosphere soil were significantly higher than that of the CK. There were 6 main phyla in the fungal community while the most dominant fungi phylum was Ascomycete in the 3-season and 5-season continuous cropping soil. Arthrobotyrs was dominant in 3-season continuous cropping soil while Pepziza was dominant in that of 5-season continuous cropping. The principle component analysis of continuous cropping soil revealed that there were similar Mycoflora communities and quantities between the soil of CK and 3-season continuous cropping, which was significantly different from that of the 5-season continuous cropping. (3) There was a correlation between potato autotoxins and rhizosphere fungi communities and quantities. The abundances of syringic acid and Alternaria, vanillic acid and Peziza had significantly positive relation, the abundances of ferulic acid and Peziza had significantly negative relation. Therefore, autotoxins accumulated constantly after continuous cropping of potatoes in the rhizosphere soil, it altered the structure of fungal communities and amassed the harmful fungi such as Alternaria and Fusarium.