玉米热激蛋白ZmHSP70-12基因的克隆及表达分析

热激蛋白70(HSP70)是原核和真核细胞中普遍存在的一种高度保守的分子伴侣。从玉米中克隆1个HSP70家族成员。该基因cDNA序列全长为1 992 bp,开放阅读框为2 352 bp,编码663个氨基酸,蛋白质分子量约75.0 kD。蛋白结构预测及同源比对分析表明,该基因编码蛋白含ATPase位点和HSP70保守结构域,与拟南芥AtHSP70-12序列高度相似,命名为ZmHSP70-12。蛋白亚细胞定位显示,ZmHSP70-12蛋白在内质网中表达。实时荧光定量PCR分析表明,ZmHSP70-12对非生物胁迫高温、干旱均具有明显的应答反应,推测ZmHSP70-12是玉米中与胁迫逆境相关的基因。

Cloning and Expression Analysis of Heat Shock Protein Gene ZmHSP70-12 in Maize

Heat shock protein 70(HSP70s) are found extensively in prokaryotic and eukaryotic and one of the most conserved molecular chaperones. In this study, a member of the HSP70 family was isolated from maize. Its fulllength coding sequence was 1 992 bp, and its open reading frame is 2 352 bp. The encoded protein with molecular weight is about 75.0 kD. Through protein structure prediction and homologous alignment analysis, the gene encodes a protein containing ATPase site and HSP70 conserved domain. It is highly similar to Arabidopsis thaliana AtHSP70-12 sequence, so it is named ZmHSP70-12. Subcellular localization analysis showed that the ZmHSP70-12 protein was expressed in the endoplasmic reticulum. Real-time quantitative PCR analysis revealed ZmHSP70-12 has an obvious response to abiotic stress, such as high temperature and drought. Therefore, it speculates that ZmHSP70-12 is a stress-related gene in maize stress.