小鼠日粮中添加小花棘豆黄酮的安全性试验

试验旨在研究小鼠日粮中添加不同剂量的小花棘豆黄酮在其饲喂过程中的安全性。选用48只6~8周龄、体重为(20±2)g的小鼠,随机分为4组,每组12只,雌、雄各半。对照组饲喂全混合日粮,试验Ⅰ、Ⅱ、Ⅲ组在全混合日粮中分别添加10、30、50 mg/kg小花棘豆黄酮,试验期为30 d。结果表明,小鼠采食小花棘豆黄酮后未见异常临床症状;小鼠血液学指标、血清生化指标和脏器系数均在正常范围内。提示,小鼠日粮中添加小花棘豆黄酮未发现亚慢性毒性,可以被安全利用。

Safety Evaluation of Adding Flavonoid from Oxytropis Glabra DC in Forage for Mice

The experiment was aim to evaluate the clinical safety on feeding the different does of flavonoid from Oxytropis glabra DC in diet for mice. 48 mice aged from 6-week-old to 8-week-old were randomly divided into 4 groups,the average body weight of mice was (20±2)g, there were 12 mice in every group, every group had 6 male mice and 6 female mice.The control group was fed complete feed,the experimentⅠ,Ⅱ,Ⅲ groups were fed with the adding of 10,30,50 mg/kg flavonoid from Oxytropis glabra DC.The trial period was 30 d. The results revealed that fed with flavonoid from Oxytropis glabra DC had no any abnormal clinical symptoms.The serum biochemical indicators,hematological indices and organ coefficients exsited in normal limits. It showed that flavonoid from Oxytropis glabra DC in forage had no subchronic toxicity, and was safety for mice.