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Effect of Environmental Stress and Ethylene on the Expression of LeACO1 Gene
作者姓名:HU Zong-li  CHEN Guo-ping  LV Li-juan  CHEN Xu-qing  YAN Bo
作者单位:College of Bioengineering, Chongqing University, Chongqing,400030, China
摘    要:The specific ACC oxidase 1 gene fragment of tomato was cloned and prepared for a probe which was used to hybridize with total RNAs that were extracted from the materials treated with ethylene and different environmental stresses, such as drought, water recovery, flood, wound and temperature, respectively. Northern blot analysis indicated that the expression of LeACO1 was restrained by drought, while strongly induced by water recovery and exogenous ethylene. After treated by flood stress, the expression level of I_eACO1 in leaves had no distinctively changes, while its expression level in stems was rapidly increased. Moreover, the expression of LeACO1 in tomato stems could be induced at the temperature of 37 degree. In addition, wounding had no influence on the expression of LeACO1.

关 键 词:environmental  stress  ethylene  ACC  oxidase  1(ACO1)  tomato
修稿时间:2006/8/10 0:00:00

Effect of Environmental Stress and Ethylene on the Expression of LeACO1 Gene
HU Zong-li,CHEN Guo-ping,LV Li-juan,CHEN Xu-qing,YAN Bo.Effect of Environmental Stress and Ethylene on the Expression of LeACO1 Gene[J].Storage & Process,2007(1):129-133.
Authors:HU Zong-li  CHEN Guo-ping  LV Li-juan  CHEN Xu-qing  YAN Bo
Institution:College of Bioengineering, Chongqing University, Chongqing,400030, China
Abstract:The specific ACC oxidase 1 gene fragment of tomato was cloned and prepared for a probe which was used to hybridize with total RNAs that were extracted from the materials treated with ethylene and different environmental stresses, such as drought, water recovery, flood, wound and temperature, respectively. Northern blot analysis indicated that the expression of LeACO1 was restrained by drought, while strongly induced by water recovery and exogenous ethylene. After treated by flood stress, the expression level of I_eACO1 in leaves had no distinctively changes, while its expression level in stems was rapidly increased. Moreover, the expression of LeACO1 in tomato stems could be induced at the temperature of 37 degree. In addition, wounding had no influence on the expression of LeACO1.
Keywords:environmental stress  ethylene  ACC oxidase 1(ACO1)  tomato
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