|
|||||
|
|
| 绿色荧光蛋白(GFP)基因在Pseudozyma antarctica中的表达 | |
| 引用本文： | 赵颖怡,成亚力,梁世中. 绿色荧光蛋白(GFP)基因在Pseudozyma antarctica中的表达[J]. 广西农业生物科学, 2005, 24(1): 14-17 |
| 作者姓名： | 赵颖怡 成亚力 梁世中 |
| 作者单位： | 1. 华南理工大学,生命科学院,广东,广州,510640;加拿大Laval大学,生物控制系,Quebec Province,GEK 7P4;广西大学轻工与食品工程学院,广西,南宁,530004 2. 加拿大Laval大学,生物控制系,Quebec Province,GEK 7P4 3. 华南理工大学,生命科学院,广东,广州,510640 |
| 摘 要： | Pseudozyma antarctica隶属于担子菌纲。P．antarctica的双质粒共转化系统包括：pSceI-hyg和pVectour libre，其中pSce I-hyg含有hsp70启动子和终止子以及潮霉素抗性基因；pVectour libre仅含有hsp70启动子和终止子，以便插入要表达的目的基因。设计引物扩增得到绿色荧光蛋白基因后，插入pVectour libre，构建质粒GFP．hsp，将该质粒与pSce I-hyg共转化P．antarctica；实现了该系统在P．antarctica中的绿色荧光表达。 |
| 关 键 词： | 绿色荧光蛋白 担子菌 表达 |
| 文章编号： | 1008-3464(2005)01-0014-04 |
| 修稿时间： | 2003-10-20 |
Expression of green fluoresent protein(GFP) in Pseudozyma antarctica |
|
| ZHAO Ying-yi,CHENG Ya-li,Rihcard Belanger,LIANG Shi-zhong. Expression of green fluoresent protein(GFP) in Pseudozyma antarctica[J]. Journal of Guangxi Agricultural and Biological Science, 2005, 24(1): 14-17 | |
| Authors: | ZHAO Ying-yi CHENG Ya-li Rihcard Belanger LIANG Shi-zhong |
| Affiliation: | ZHAO Ying yi1,2,3,CHENG Ya li2,Rihcard Belanger2,L IANG Shi zhong1 |
| Abstract: | Pseudozyma antarctica is Basidiomycetes speciesbelonging to filamentousfungi.The co- transformation system of P. antarctica includes two plasmids:p Sce I-hyg and p Vectour libre.The plasmid p Sce I-hyg consists of hsp70 promoter,hygromicine gene and hsp70 terminator while the plasmid p Vectour libre only contains hsp70 promoter and terminator in order to be inserted by interesting genes.The specific gene primers were designed based on green fluoresent protein ( GFP) sequence,then GFP gene was obtained by PCR,and inserted into p Vectour libre to achieve the recombinantvector GFP.hsp,which was ready for the co-transformation with p Sce I-hyg.Specific fluorescence can be observed from co-transformation system in P.antarctica using a fluorescent microscope. |
| Keywords: | green fluorescentprotein ( GFP) Pseudozymeantarctica expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |