| 应用组织芯片免疫组化法检测ALV-J |
| |
| 引用本文： | 张玲娟,刘杰,成子强,王桂花,刁秀国,朱国. 应用组织芯片免疫组化法检测ALV-J[J]. 中国预防兽医学报, 2006, 28(4): 427-430 |
| |
| 作者姓名： | 张玲娟 刘杰 成子强 王桂花 刁秀国 朱国 |
| |
| 作者单位： | 山东农业大学,动物科技学院,山东,泰安,271018 |
| |
| 摘 要： | 禽白血病J亚群（ALV-J）是英国的Payne和他的同事们在20世纪90年代初从肉鸡中分离出来的新亚群，主要引起肉鸡的骨髓瘤白血病。自1999年，我国一些肉用型种鸡场陆续发生了禽白血病J亚群。并且近几年来，ALV-J已从最初只引起肉种鸡发病开始向蛋鸡及中国地方种鸡蔓延。组织芯片技术是一种新型特殊的生物芯片技术，它能明显提高工作效率，减少实验误差。本研究将组织芯片技术和免疫组化染色结合起来，用特异性抗ALV-J囊膜蛋白gp85的单克隆抗体来检测发病鸡只的各组织器官的组织切片。在肝脏、脾脏、肾脏、卵巢、腺胃、骨髓、髓细胞瘤组织均检出病毒阳性抗原。结果表明组织芯片技术和免疫组化染色相结合为临床诊断ALV-J提供了一个高通量、敏感的检测方法。
|
| 关 键 词： | 组织芯片 免疫组化 禽白血病J亚群(ALV-J) |
| 文章编号： | 1008-5089(2006)04-0427-04 |
| 收稿时间： | 2005-05-14 |
| 修稿时间： | 2005-05-14 |
Detecting avian leukosis virus subgroup J using tissue chip and immunohistochemical technology |
| |
| ZHANG Ling-juan,LIU Jie,CHENG Zi-qiang,WANG Gui-hua,DIAO Xiu-guo,ZHU Guo. Detecting avian leukosis virus subgroup J using tissue chip and immunohistochemical technology[J]. Chinese Journal of Preventive Veterinary Medicine, 2006, 28(4): 427-430 |
| |
| Authors: | ZHANG Ling-juan LIU Jie CHENG Zi-qiang WANG Gui-hua DIAO Xiu-guo ZHU Guo |
| |
| Affiliation: | College of Animal and Science of Shandong Agriculture University, Taian 271018, China |
| |
| Abstract: | Avian Leukosis Virus(ALV-J)is a retrovirus that infects meat-type chickens.ALV-J was reported as a new subgroup of ALV by Payne in 1991.It induced avian myelcytomatosis(ML)in broiler.ALV-J has been spread in our country since 1999.Now ALV-J begins to infect egg-laying hens and Chinese local chickens.The tissue chip is a new special biological chip.It can improve efficiency and avoid error.This study connects tissue chip with immunohistochemical technology.Positive antigens were detected in checked slides with special monoclonal antibody against gp85 of ALV-J.It revealed that this method was not only efficient but also sensitive. |
| |
| Keywords: | tissue chip immunohistochemical technology avian leukosis virus subgroup J(ALV-J) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
