利用基于HRM的功能标记分析水稻Wx和fgr的基因型

Wx和fgr基因分别调控水稻直链淀粉含量和香气形成。在基于凝胶电泳功能标记的基础上，发展基于高分辨率熔解曲线(HRM)的功能标记Wx–a/b和BAD2–E7, 分别用于鉴定88份水稻种质Wx和fgr的基因型。利用LightScanner 96平台进行HRM检测，单次检测在10 min内即可完成最多96个样品的基因型分析。结果表明，88份水稻种质中，25份含纯合Wxa基因型的种质，直链淀粉含量为25.08%；59个含纯合Wxb基因型的种质，直链淀粉含量为15.00%，其余4个杂合型(Wxa/Wxb)的直链淀粉含量为18.13%。携带纯合fgr基因的3份种质(巴太香占、象牙香占和Basmati 370)，其全部米粒均带有香气，而携带杂合fgr的2份种质，只有部分米粒带有香气。

Analysis of rice genotypes rice Wx and fgr by HRM-based functional marker

The amylose content and fragrance in rice are controlled by Wx and fgr respectively. Here, based on the gel-based FMs reported previously, we developed two HRM(high resolution melting)-based FMs Wxa/b and BAD2E7 to study the genotype of 88 rice accessions in Wx and fgr gene. We implemented the LightScanner 96 platform to determine the HRM curves, through which each determination for up to 96 samples could be done in less than 10 min. The result showed that the amylose content of 25 homozygous Wxa accessions and 59 homozygous Wxb accessions was 25.08% and 15.00%, respectively, and that of 4 heterozygous (Wxa/Wxb) accessions was 18.13%. All grains of 3 accessions (Bataixiangzhan, Xiangyaxiangzhan and Basmati 370) harboring homozygous fgr were fragrant, and only partial grains of 2 accessions with heterozygous fgr were fragrant. Thus it can be seen that the HRM-derived functional markers could be applied to rapidly and accurately predict their corresponding phenotypes, showing good application prospects in rice breeding practice.