补喂L-瓜氨酸对种公马精液品质、精清和血浆抗氧化能力的影响

【目的】 研究补喂L-瓜氨酸对配种期种公马精液品质、精清和血浆抗氧化能力的影响。【方法】 选择年龄5~8岁的种公马12匹,平均采精量为(59.34±5.23) mL,随机分为对照组和试验组2组,每组6匹。所有马匹在相同的饲养环境下,饲喂相同营养水平的日粮,试验组马匹在此基础上补喂20 g/d L-瓜氨酸。【结果】 (1)补喂L-瓜氨酸能够极显著提高采精量和精子直线运动率,比对照组分别提高28.08%(P＜0.01)和19.12%(P＜0.01);(2)补喂L-瓜氨酸能够极显著提高精清SOD活力和抑制羟自由基的能力(P＜0.01),显著提高GSH-PX的活力(P＜0.05),极显著降低MDA的浓度(P＜0.01);(3)补喂L-瓜氨酸能够极显著提高血浆CAT、GSH-PX的活力(P＜0.01)和T-AOC、抑制羟自由基的能力(P＜0.01),极显著降低MDA的浓度(P＜0.01)。【结论】 对配种期种公马补喂20 g/d L-瓜氨酸能够提高种公马精液品质和精清、血浆的抗氧化能力。

Effects of Supplementary L-citrulline on the Semen Quality and the Anti-Oxidation Ability of Seminal Plasma and Plasma of Stallion

【Objective】 The purpose of this experiment is to study the effects of supplementary L-citrulline on the semen quality and the anti-oxidation ability of seminal plasma and plasma of stallion. 【Methods】 Twelve stallions at the age of 5-8 years were selected and randomly divided into control and trial group with 6 horses in each group. All horses were bred in the same environment and fed the same nutrient level diet, the trial group horses were fed 20 g/d L-citrulline. 【Results】 (1)Compared with the control group, supplementary L-citrulline significantly increased the amount of sperm collected and the linear rate of sperm movement by 28.08% (P＜0.01) and 19.12% (P＜0.01), respectively. (2) L-citrulline could significantly improve the SOD activity and inhibit the hydroxyl radical activity of the seminal plasma (P＜0.01), significantly increased the viability of GSH-PX (P＜0.05), and significantly reduce the concentration of MDA (P＜0.01). (3) Supplementary L-citrulline could significantly increased the activity of CAT, GSH-PX, T-AOC and inhibit hydroxyl free radicals of plasma (P＜0.01), and significantly reduce the concentration of MDA (P＜0.01). 【Conclusion】 Therefore, supplementary L-citrulline fed to the breeding stallions can improve their semen quality and the anti-oxidation ability of the sperm and plasma of stallion.