茯砖茶对肠道4种常住微生物的影响

用致泻剂番泻叶建立小鼠腹泻模型，研究茯砖茶水提物高、中、低剂量(分别为210、105、52.5 mg/mL对肠道大肠杆菌、肠球菌、双歧杆菌、乳杆菌数量及肠道状况的影响。结果显示：高、中剂量茯砖茶能促进肠道有益微生物的生长，抑制有害微生物增殖，对抗番泻叶所致的肠道微生物紊乱，其中对乳杆菌增殖的影响最为显著，预防组高、中、低剂量可促进乳杆菌繁殖，试验第7天比试验开始前的数量对数值分别增加3.309 0、3.237 3、2.690 5，且呈剂量依懒关系；治疗组高剂量可促进乳杆菌的增殖，试验第11天的数量对数值比试验第7天的增加3.541 9，比阳性药小檗碱促进乳杆菌增殖的效果(对数值增加1.466 9)更好；中、低剂量可减少肠球菌数量，试验第11天的数量对数值比第7天的分别减少0.423 6、0.231 5，而阳性药小檗碱反而使肠球菌数量增加，高、中、低剂量组试验第11天的大肠杆菌数量的对数值与试验第7天的相比分别增加0.471 0、0.335 3、0.510 9，与阳性药组(0.556 2)差异较小；灌服茶汤的小鼠的肠腔状况与正常空白对照小鼠的无异样。

Effect of Fuzhuan tea on permanent intestinal microflora

The effects of different dosages (210, 105, 52.5 mg/mL) of Fuzhuan tea on quantity of Escherichia coli, Enterococcus, Bifidobacterium spp. and Lactobacillus spp. and on intestinal physiology were investigated using mice diarrhea model caused by Senna leaf. The results showed that high and middle dosage of Fuzhuan tea could not only promote the reproduction of intestinal beneficial microbes, but also restrain harmful microbes, thus preventing gut microbe disorder caused by Senna leaf. Among these effects, the growth of Lactobacillus spp. was the most affected character by Fuzhuan tea. In prevention groups with high-, middle- and low-dose Fuzhuan tea, the growth of Lactobacillus spp. was promoted, whose logarithm of bacterial number increased 3.309 0, 3.237 3 and 2.690 5 respectively on the 7th day compared with the bacterial number before treatment, indicating a dose dependent increase. High-dose Fuzhuan tea in treatment group could promote the growth of Lactobacillus spp.. Comparing the quantity of Lactobacillus spp. on the 7th d, the logarithm of bacterial number increased by 3.541 9 on the 11th d, better than berberine treated group (logarithm of the numbers of Lactobacillus spp. only increased 1.466 9). Middle- and low-dose Fuzhuan tea in treatment group could decrease Enterococcus, the logarithm of the numbers of which decreased by 0.423 6, 0.231 5 respectively, which was contrary to berberine treated group in which Enterococcus was increased. In treatment group, logarithm of the numbers of Escherichia coli on the 11th day were increased by 0.471 0, 0.335 3 and 0.510 9 respectively by high-, middle- and low-dose Fuzhuan tea compared to the quantity of Escherichia coli on the 7th day, which showed small difference compared to berberine treated group in which logarithm of the numbers of Escherichia coli increased by 0.556 2. There was no difference in intestinal characteristics between Fuzhuan tea treated mice group and the CK.