二硝基重氮酚（DDNP）废水中的硝基酚类化合物污染土壤对2种蔬菜种子萌发与生长的影响

以上海青、小白菜2种蔬菜种子为材料，研究受二硝基重氮酚 (DDNP)废水污染（以硝基酚类化合物计）的土壤对种子萌发、根或芽伸长及光合与呼吸作用强度的影响。结果表明，土壤硝基酚类化合物含量在0~12 mg/kg时，对上海青、小白菜的发芽率有不显著(P>0.05)的促进作用，超过24 mg/kg时，上海青发芽率受明显抑制，达到36 mg/kg时，受污染土壤明显抑制小白菜发芽率；2种蔬菜根和芽伸长抑制率与污染土壤中硝基酚类化合物含量呈显著线性相关（P<0.01），上海青根和芽伸长的IC₅₀分别为60.39、99.34 mg/kg，小白菜根及芽伸长的IC₅₀分别为65.48、109.89 mg/kg，对于硝基酚类化合物污染，上海青较小白菜更敏感，对同一种作物，根较芽更敏感；随着土壤中硝基酚类化合物含量的增大，2种蔬菜光合作用所利用的CO₂量及呼吸作用所产生的CO₂量均呈下降趋势，DDNP废水污染土壤会影响植物光合及呼吸作用，引起机体受损。

Effect of the Soil with Pollution of Dinitrodiazophenol (DDNP) Wastewater with the Itrophenols on the Seed Germination and Growth of Two Vegetables

With two common vegetables (Shanghaiqing ,Pakchoi) seeds as materials ,the effect of the soil with pollution of DDNP wastewater pollution(by the concentration of nitrophenols) on their seed germination, root and shoot elongation inhibition, the strength of photosynthetic and respiration are studied. Results demonstrated that in the range of 0-12 mg/kg the nitrophenols pollution could promote the seed germination rate of the two plants, but did not reach the significance level (P>0.05). With the concentration over 24 mg/kg, the germination rate of Shanghaiqing could be inhibited, and as it was more than 36 mg/kg, the Pakchoi germination rate of could be inhibited. Their was a significant (P<0.01) linear relationship between the concentration of nitrophenols within the soil with the root and shoot elongation inhibition rate of the two vegetables. The IC₅₀ of the root and shoot elongation inhibition rate of Shanghaiqing was 60.39 and 99.34 mg/kg, and the IC₅₀ of the Pakchoi was 65.48 and 109.89 mg/kg. For the nitrophenols pollution, Shanghaiqing is more sensitive, to the same kind of crops, the root is more sensitive to shoot. As the increase of nitrophenols concentration within the soil, the amount of CO₂ used by photosynthetic and produced by respiration was downtrend. It shows that the pollution of DDNP wastewater could influent the photosynthetic and respiration and caused damage of the plant organism.