转移因子对猪圆环病毒亚单位疫苗免疫效果的影响

为探寻转移因子(TF)对猪圆环病毒亚单位疫苗免疫效果的影响，将猪脾脏研磨，并反复冻融研磨液，离心获取冻融上清，用超滤法从上清液中提取转移因子。TF经理化检验合格后，以添加剂的形式与猪圆环病毒亚单位疫苗(Cap疫苗)混合，制成TF–Cap疫苗，并和未添加TF的Cap疫苗同时进行小鼠免疫试验。TF–Cap疫苗和Cap疫苗免疫的小鼠都设一免组和二免组，首次免疫后每隔10 d对实验鼠进行尾静脉采血，并用间接ELISA方法测定血清中猪圆环病毒2型(PCV2)的抗体水平。结果表明：用超滤法制得的TF，多肽含量为1.83 mg/mL；抗体检测数据显示，4个免疫组与对照组(注射生理盐水)的抗体检测值有极显著差异(P<0.01)，而4个免疫组之间无显著差异(P>0.05)，TF–Cap疫苗一免组的抗体水平与Cap疫苗二免组的抗体水平相当，表明在圆环病毒基因工程疫苗中添加TF可一定程度的提高免疫动物的抗体水平。

Effect of transfer factor on immune efficacy of PCV2 subunit vaccine

To investigate the effect of transfer factor (TF) on the immune efficacy of porcine circovirus type 2 (PCV2) subunit vaccine, spleen from healthy porcine was grinded and the homogenate was frozen thawed six times. TF was obtained after the homogenate was centrifuged and ultrafiltered. After physical and chemical inspection, TF was added to PCV2 subunit vaccine (Cap vaccine), named TF-Cap vaccine. TF-Cap vaccine and Cap vaccine were used to immunize mice with physiological saline as control. The mice were separated into two groups: one-time vaccination and two-time vaccination. Blood was collected at 10-d interval through caudal vein after the first vaccination and PCV2 antibody was tested using indirect enzyme-linked immuno-sorbent assay. The result showed that the concentration of the ultrafiltered TF is 1.83 mg/mL. Detection of PCV2 specific antibody showed that there are significant differences (P<0.01) between vaccine group and control group while there is no significant differences (P>0.05) among vaccine groups. However, PCV2 antibody titer in mice immunized TF-Cap vaccine only once was comparable to mice immunized Cap-vaccine twice, indicating PCV2 subunit vaccine with TF can enhance the antibody production.