贺兰山紫蘑体外抗氧化活性的初步研究

为评价贺兰山紫蘑（Cortinarius rufo olivaceus）的抗氧化活性,以紫红丝蘑菌为原料,通过甲醇超声震荡提取,经正己烷、乙酸乙酯、正丁醇依次进行萃取,测定各萃取相的总抗氧化能力、清除DPPH自由基能力、抑制羟基自由基能力及抗超氧阴离子自由基能力。结果表明,贺兰山紫蘑各萃取相具有清除自由基的能力,在同一质量浓度水平下正丁醇相和水相具有较高的抗氧化活性,试验结论为研究贺兰山紫蘑的保健作用机理提供参考,并为其开发利用提供理论依据。

Study on Antioxidant Activity of Cortinarius rufo olivaceus

In order to evaluate the antioxidant activity of Cortinarius rufo olivaceus, the Cortinarius rufo olivaceus(Pers.)Fr. was used as material and extracted with ultrasonic methanol, and the extracts were partitioned with Hexane, EtOAc and BuOH successively, then antioxidant activities of the extracts were evaluated, including the total antioxidant capacity, the 1,1 diphenyl 2 picrylhydrazyl (DPPH) radical scavenging ability, the ability to inhibit hydroxyl radical and the ability of anti superoxide anion radical. The results showed that the extracts of Cortinarius rufo olivaceus had a certain antioxidant, and antioxidant activities of BuOH and water extracts were higher at the same concentration levels compared with others. The study provides the health function mechanism of Cortinarius rufo olivaceus for its development and utilization.