| 南抗杨叶片快速繁殖体系的建立 |
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| 引用本文： | 杨茹,项艳. 南抗杨叶片快速繁殖体系的建立[J]. 安徽农业大学学报, 2011, 38(2): 208-211. DOI: CNKI:34-1162/S.20110223.1639.015 |
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| 作者姓名： | 杨茹 项艳 |
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| 作者单位： | 安徽农业大学林学与园林学院,合肥,230036;安徽农业大学林学与园林学院,合肥,230036 |
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| 基金项目： | 国家科技支撑计划子课题，安徽省科技攻关重大专项 |
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| 摘 要： | 以南抗杨叶片为材料,对其快繁体系进行研究。选用9种分化培养基、4种增殖培养基和6种生根培养基,研究不同激素组合对快速繁殖体系建立的影响。结果表明,适宜的诱导分化培养基为MS+6-BA 1.5 mg·L-1+NAA 0.5 mg·L-1+KT 0.1 mg·L-1;丛生芽增殖培养基为MS+6-BA 1.2 mg·L-1+NAA 0.3 mg·L-1+KT 0.1 mg·L-1;最佳生根培养基为1/2MS+IBA 0.5 mg·L-1+NAA 0.01 mg·L-1。
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| 关 键 词： | 南抗杨 组织培养 快繁体系 |
| 收稿时间： | 2010-08-31 |
Establishment of fast regeneration system from leaves of Populus deltoides cl. Nankang |
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| YANG Ru and XIANG Yan. Establishment of fast regeneration system from leaves of Populus deltoides cl. Nankang[J]. Journal of Anhui Agricultural University, 2011, 38(2): 208-211. DOI: CNKI:34-1162/S.20110223.1639.015 |
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| Authors: | YANG Ru and XIANG Yan |
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| Affiliation: | (School of Forestry and Landscape Architecture,Anhui Agricultural University,Hefei 230036) |
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| Abstract: | This paper developed fast reproduction system from leaves of Populus deltoides cl.Nankang with 9 differentiation media,4 multiplication media and 6 rooting media. The effect of hormone concentration was also studied. The results indicated that the best differentiation media was MS + 6-BA 1.5 mg·L-1 + NAA 0.5 mg·L-1 + KT 0.1 mg·L-1; the best multiplication media was MS + 6-BA 1.2 mg·L-1 + NAA 0.3 mg·L-1 + KT 0.1 mg·L-1; the best rooting media was 1/2MS + IBA 0.5 mg·L-1 + NAA 0.01 mg·L-1. |
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