绒毛狼尾草幼穗的愈伤组织诱导与植株再生

以绒毛狼尾草幼嫩花穗为材料,研究了培养基种类和2,4-D对愈伤组织诱导以及6-BA对分化的影响。结果表明,MS较N6培养基适合绒毛狼尾草的组织培养。在附加3 mg·L-1 2,4-D的MS培养基中愈伤组织诱导率最高,达到100%。分化培养基以附加1.0 mg·L-1 6-BA的MS培养基为最佳,分化率为44.0%。在1/2MS培养基中附加0.5 mg·L-1 NAA和0.3 mg·L-1活性炭进行生根培养,根系生长健壮,生根率达100%。

Callus induction and plant regeneration from young spike of Pennisetum setaceum

The effects of media types and concentration of 2,4-D on callus-induction with young spike of Pennisetum setaceum, and some concentrations of 6-BA on callus differentiation were studied. The result shows MS is the optimal medium on callus-induction. The callus induction rate reached its maximum of 100% at MS media with 3 mg·L^-1 2,4-D, in the differentiation media with 1.0 mg·L^-1 6-BA, the optimum differentiation rate was achieved at 44.0%, and in the 1/2 MS media plus 0.5 mg·L^-1 NAA and 0.3 mg·L^-1 activated charcoal, root generation rate reached 100%.