西岛内港沉积物放线菌的分离及其抗菌活性研究 |
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引用本文: | 徐小雄,冯慧敏,陈永敢,马红梅.西岛内港沉积物放线菌的分离及其抗菌活性研究[J].现代农业科技,2011(11):17-18. |
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作者姓名: | 徐小雄 冯慧敏 陈永敢 马红梅 |
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作者单位: | 琼州学院;海南省两栖爬行动物重点实验室(筹); |
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基金项目: | 三亚市院地科技合作项目(2010YD21); 琼州学院青年基金项目(QYQN201134) |
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摘 要: | 从西岛内港采集沉积物,采用自然风干后,湿热60℃、处理10 min的预处理方法,稀释涂布于GA培养基上,成功地分离得到44株海洋源的放线菌。经初步形态显微观察判断,其中18株为链霉菌,26株为非链霉菌,链霉菌中以小单孢菌为主。18株链霉菌抗香蕉枯萎病测试结果显示,菌株090314表现出一定的抗香蕉枯萎病菌(4号生理小种,FOC 4)活性。
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关 键 词: | 沉积物 放线菌 分离 抗菌活性 西岛内港 |
Study on Selective Isolation and Anti-FOC 4 Activity of Actinobacteria from Sediment of Xidao Wharf |
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Authors: | XU Xiao-xiong FENG Hui-min CHEN Yong-gan MA Hong-mei |
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Institution: | XU Xiao-xiong 1,2 FENG Hui-min 1 CHEN Yong-gan 1 MA Hong-mei 1(1 Qiongzhou University,Sanya Hainan 572022,2 Hainan Key Laboratory for Herpetological Research(preparation)) |
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Abstract: | After pretreatments of steam heat(60 ℃,10 min),the sediment suspensions collected from the wharf of Xidao island were transferred and spread onto the GA media.44 actinobacteria including 18 Streptomyces and 26 non-Streptomyces-like were isolated according to their morphology on agar plates and under microscope.Strain 090413 revealed antagonistic effect of against Fusarium oxysporum f.sp.cubense(race 4,FOC 4). |
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Keywords: | sediment actinobacteria Isolation anti-FOC 4 activity Xidao wharf |
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