Effect of CoQ10 on apoptosis and proliferation of cultured mouse microvascular endothelial cells and it's mechanism |
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| Authors: | ZHANG Guo-ping YUE Fei JIN Hui-ming SHEN Hao Masao MORI WANG Zhong |
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| Institution: | 1. Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China;
2. Shanghai People's Hospital No.6, Shanghai 200233, China;
3. Wakan Shoyaku Botany Institute, Tokyo, Japan |
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| Abstract: | AIM: To study the inhibitory effect of CoQ10 on the apoptosis of microvascular endothelial cells and it's probable mechanism. METHODS: Using serum pharmacology method and cytoflowmetery, the effects of CoQ10 at different concentrations on apoptosis and proliferation in cultured mouse brain microvascular endothelial cells (bEnd.3) were investigated. The expression of Fas protein and Bcl-2 protein were observed with immunocytochemical method (ABC). RESULTS: The cell apoptosis was inhibited significantly in CoQ10 groups (50 μL and 25 μL) in cultured bEnd.3 cells. The results of immunocytochemical staining showed that the expressions of Fas protein was inhibited and Bcl-2 protein was stimulated significantly in CoQ10 group with above concentration. But there was no significant change in cell proliferation. CONCLUSIONS: CoQ10 may inhibit apoptosis of microvascular endothelial cells (bEnd.3) via up-regulation of Bcl-2 and down-regulation of Fas. Authors suggest that this is one of the protection mechanisms of CoQ10 from dysfunction of microvascular endothelial cells. |
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| Keywords: | Ubiquinone Apoptosis Brain Endothelial cells Cell proliferation Bcl-2 protein Fas protein |
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