Effect of isoflavone genistein on activation and proliferation of mouse T cells in vitro |
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Authors: | WANG Qing HE Xian-hui ZHAO Jing-xian YANG Zhi-gang ZENG Yao-ying |
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Institution: | Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632, China |
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Abstract: | AIM: To study the effect of genistein on activation and proliferation of T cells, and explore the molecular mechanism of genistein. METHODS: Fluorescence conjugated monoclonal antibodies and flow cytometry were used to detect the express of CD69 and CD25 by activated T cells in vitro in response to Concanavalin (ConA )and Phorbol 12, 13-dibutyrate(PDB) or T cell proliferation stained by CFSE in response to PDB / Ionomycin or ConA. RESULTS: Genistein inhibited the expression of CD69 and CD25 in activated T cells in response to Con A in a concentration-dependent manner and in response to PDB in a high concentration. Genistein inhibited proliferation of T cells in both groups in a concentration-dependent manner. CONCLUSION: Genistein inhibited activation and proliferation of T cells in vitro in response to polyclonal stimulus, and it may hold potential as a new immunosuppressant. |
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Keywords: | Genistein Antigen CD69 Receptors interleukin-2 T-lymphocytes Flow cytometry |
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