| 农杆菌介导获得高亲和NH_4~+转运因子转基因甜菜 |
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| 引用本文： | 郇志荣,孙亚卿,李国龙,刘景秀,张少英.农杆菌介导获得高亲和NH_4~+转运因子转基因甜菜[J].分子植物育种,2010,8(2). |
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| 作者姓名： | 郇志荣 孙亚卿 李国龙 刘景秀 张少英 |
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| 作者单位： | 1. 内蒙古农业大学甜菜生理研究所,呼和浩特,010018
2. 内蒙古农业大学生态环境学院,呼和浩特,010018 |
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| 基金项目： | 现代农业产业技术体系建设专项资金项目 |
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| 摘 要： | 本研究利用农杆菌介导的方法将高亲和NH4+转运因子融合基因转入甜菜子叶节外植体。遗传转化过程主要包括子叶节预培养、侵染转化、共培养、洗菌、抑菌培养、潮霉素筛选分化培养、抗性苗生根移栽。实验获得抗性苗140株,抗性芽分化率为8.14%,随机抽取24株抗性苗进行PCR检测,获得转基因植株10株。
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| 关 键 词： | 甜菜 农杆菌介导 高亲和NH+4 转运因子 遗传转化 融合基因 |
Transgenic Sugar Beet Harbouring High-affinity NH4+ Transporter Gene Acquired by A grobacterium-mediated Transformation |
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| Huan Zhirong,Sun Yaqing,Li Guolong,Liu Jingxiu,Zhang Shaoying.Transgenic Sugar Beet Harbouring High-affinity NH4+ Transporter Gene Acquired by A grobacterium-mediated Transformation[J].Molecular Plant Breeding,2010,8(2). |
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| Authors: | Huan Zhirong Sun Yaqing Li Guolong Liu Jingxiu Zhang Shaoying |
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| Abstract: | In this study,high-affinity NH4+ transporter fusion gene was successfully transformed into the cotyledon node explants of sugar beet by using Agrobacterium-mediated transformation methord.Genetic transformation process includes cotyledon node pre-culture,infection transformation,co-culture,washed bacteria,bacteriostatic culture,differentiation culture of hygromycin selection,and resistance seedlings to root transplanting.As the experimental result,140 resistant seedlings were obtained and its differentiation rate was 8.14%,24 resistant seedlings were selected randomly to PCR detecte,and PCR assaying of plant DNA confirmed 10 transgenic plants. |
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| Keywords: | Sugar beet (Beta Vulgaris) Agrobacterium-mediated High-affinity NH4+ transporter Genetic transformation Fusion gene |
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