利用96孔板建立除草剂微量活体筛选方法初探

以马唐、稗草、反枝苋3种杂草为指示植物,采用96孔板建立了化合物除草活性微量活体筛选方法。从6种培养基中筛选出最适合3种指示杂草生长的Ⅰ号培养基,以传统盆栽法为对照,进行了96孔板苗前处理微量活体筛选方法(以下简称96孔板法)的研究。结果表明:96孔板法以指示杂草的株高受抑制情况作为评价指标较为合适;3种供试除草剂采用96孔板法对3种指示杂草所表现出的杀草活性要高于盆栽法;乙氧氟草醚、二甲戊灵、氟乐灵盆栽法的EC50和EC90值分别是96孔板法的1.44~21.47倍和1.47~32.84倍;采用96孔板法,乙氧氟草醚对马唐和反枝苋、二甲戊灵对稗草的活性最高,EC50值分别为0.79、0.73和0.45 μg/mL,EC90值也分别只有3.13、1.72和1.36 μg/mL。96孔板法达到了微量、敏感、节省时间和空间的要求,是发现先导化合物除草活性较适宜的微量活体筛选方法。

Preliminary Study on an in vivo Micro-screening Method for Herbicidal Activity Using 96-well Plates(1. Key Laboratory of Pesticide Chemistry & Application Technology, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China;

Using Digitaria sanguinalis(L.) Scop,Echinochloa crus-galli(L.) Beauv.and Amaranthus retroflexus L.as indicator plants,an in vivo micro-screening method for the herbicidal activity was studied.Firstly,medium I was selected from six mediums,which was the most suitable one for growth of those indicator weeds.Secondly,96-well plates test was conducted as an in vivo micro-screening method comparing with traditional pot experiment.The results showed that the most suitable evaluation index for 96-well plates test was the inhibition of plant height.The herbicidial activity with 96-well plates test was more sensitive than that of pot experiment.The EC_(50)and EC_(90) value of oxyfluorfen,pendimethalinandtrifluralintoweedsbypotexperimentwere1.44-to21.47-foldsand1.47- to