New Sorbicillinoids with Tea Pathogenic Fungus Inhibitory Effect from Marine-Derived Fungus Hypocrea jecorina H8 |
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Authors: | Shun-Zhi Liu Guang-Xin Xu Feng-Ming He Wei-Bo Zhang Zhen Wu Ming-Yu Li Xi-Xiang Tang Ying-Kun Qiu |
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Institution: | 1.State Key Laboratory of Cellular Stress Biology, School of Pharmaceutical Sciences, Xiamen University, South Xiang-An Road, Xiamen 361102, China; (S.-Z.L.); (F.-M.H.); (M.-Y.L.);2.Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography State, Ministry of Natural Resources, Da-Xue Road, Xiamen 361005, China; (G.-X.X.); (Z.W.);3.State Key Laboratory of Marine Life, Ocean University of China, Yu-Shan Road, Qingdao 266100, China; |
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Abstract: | Four new dimeric sorbicillinoids (1–3 and 5) and a new monomeric sorbicillinoid (4) as well as six known analogs (6–11) were purified from the fungal strain Hypocrea jecorina H8, which was obtained from mangrove sediment, and showed potent inhibitory activity against the tea pathogenic fungus Pestalotiopsis theae (P. theae). The planar structures of 1–5 were assigned by analyses of their UV, IR, HR-ESI-MS, and NMR spectroscopic data. All the compounds were evaluated for growth inhibition of tea pathogenic fungus P. theae. Compounds 5, 6, 8, 9, and 10 exhibited more potent inhibitory activities compared with the positive control hexaconazole with an ED50 of 24.25 ± 1.57 µg/mL. The ED50 values of compounds 5, 6, 8, 9, and 10 were 9.13 ± 1.25, 2.04 ± 1.24, 18.22 ± 1.29, 1.83 ± 1.37, and 4.68 ± 1.44 µg/mL, respectively. Additionally, the effects of these compounds on zebrafish embryo development were also evaluated. Except for compounds 5 and 8, which imparted toxic effects on zebrafish even at 0.625 μM, the other isolated compounds did not exhibit significant toxicity to zebrafish eggs, embryos, or larvae. Taken together, sorbicillinoid derivatives (6, 9, and 10) from H. jecorina H8 displayed low toxicity and high anti-tea pathogenic fungus potential. |
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Keywords: | Hypocrea jecorina H8 trichodermolide C D tea pathogenic fungus inhibitory effect toxicity assessment |
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