Effects of Structural and Compositional Changes of Nanochloropsis oceania after Enzyme Treatment on EPA-Rich Lipids Extraction |
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Authors: | Kangyu Zhao Meilan Zhang Hua Tian Fenfen Lei Dongping He Jingcheng Zheng Liwei Zhang |
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Institution: | 1.School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China; (K.Z.); (M.Z.); (H.T.); (F.L.); (D.H.);2.Grain and Oil Resources Comprehensive Exploitation and Engineering Technology Research Center of State of Administration of Grain, Wuhan 430023, China |
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Abstract: | Improved methods for the extraction of eicosapentaenoic acid (EPA), an essential and economically important polyunsaturated fatty acid, are urgently required. However, lipid extraction rates using food-grade solvents such as ethanol are usually low. To improve the ethanol-based extraction rate, and to elucidate the relevant mechanisms, we used cellulase and laccase to treat powdered Nannochloropsis, one of the most promising microalgal sources of EPA. Cellulase and laccase synergistically increased lipid yields by 69.31% and lipid EPA content by 42.63%, by degrading the amorphous hemicellulose and cellulose, improving crystallinity, and promoting the release and extraction of lysodiacylglyceryltrimethylhomoserine. Scanning electron microscopy showed that cell morphology was substantially altered, with cell-wall rupture, loss of cell boundaries, and the release of intracellular substances. In conclusion, Nannochloropsis lipid yields may be directly linked to cell-wall hemicellulose structure, and enzymatic treatment to alter this may improve lipid yields. |
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Keywords: | enzyme treatment Nannochloropsis microalgae lipidomics structural and composition alteration cell wall cellulase laccase eicosapentaenoic acid betaine lipid |
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