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Production of a desirable Brassica oleracea CMS line using an alloplasmic B. rapa CMS line carrying Diplotaxis erucoides cytoplasm as a bridge plant
Authors:Yoshiaki Fujita  Keita Sunaga  Su‐Hyeun Shim  Wataru Yamada  Takayuki Ohnishi  Sang Woo Bang
Affiliation:1. Laboratory of Plant Breeding, School of Agriculture, Utsunomiya University, Utsunomiya, Japan;2. United Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Japan;3. Center for Education and Research of Community Collaboration, Utsunomiya University, Utsunomiya, Japan;4. JST, PRESTO, Kawaguchi‐shi, Japan
Abstract:In Brassica oleracea, production of F1 hybrid seeds mainly makes use of the improved Ogura cytoplasmic male sterile (CMS) line. However, reliance on one particular line is a risk, and it would be advantageous to develop other CMS lines. In this study, we transferred Diplotaxis erucoides cytoplasm to B. oleracea cultivars using an alloplasmic B. rapaCMS line as a bridge plant to avoid incompatibility between donor and recipient plants. The new B. oleraceaCMS lines, which were derived by four generations of backcrossing, had small rudimentary anthers with no pollen grain and showed complete male sterility. There was no functional defect in other floral organs, and the ability to receive normal pollen did not appear to be impaired. Moreover, the B. oleraceaCMS lines carrying D. erucoides cytoplasm had larger leaf areas and a normal plastochron. As a consequence, the B. oleraceaCMS lines carrying D. erucoides cytoplasm have the potential to be valuable alternatives for use in commercial B. oleracea hybrid seed production.
Keywords:alloplasmic line     Brassica oleracea     Brassicaceae  bridge plant  cytoplasmic male sterility     Diplotaxis erucoides     intergeneric hybridization
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