Temperature-dependent index of mitotic interval τ0 in greenling Hexagrammos otakii |
| |
| Authors: | In-Seok Park Eun-Mi Kim Seon Rang Woo Sung-Yong Oh Dong Soo Kim Jun Wook Hur |
| |
| Institution: | Division of Marine Environment and Bioscience, College of Ocean Science and Technology, Korea Maritime University, 1 Dongsam-Dong, Youngdo-Gu, Busan 606-791,;Laboratory of Molecular Oncology, Korea Cancer Center Hospital, 215-4 Gongneung-Dong, Nowon-Gu, Seoul 139-706,;Marine Living Resources Research Division, Korea Ocean Research &Development Institute, Seoul 425-600,;Department of Aquaculture, College of Fisheries Science, Pukyong National University, 599-1 Daeyeon-3 Dong, Nam-Gu, Busan 608-737, and;Research Institute of Marine Science and Technology, Korea Maritime University, 1 Dongsam-Dong, Youngdo-Gu, Busan 606-791, South Korea |
| |
| Abstract: | ABSTRACT: The aim of this study was to establish effective procedures for chromosome manipulation in greenling Hexagrammos otakii Jordan et Starks, which has enormous aquacultural potential. To accomplish this, temperature-dependent measurements of the mitotic intervals (τ0) were carried out. The τ0 in this fish was determined by averaging the duration of the first and third embryonic divisions at temperatures ranging 5–25°C. At higher temperatures, eggs developed faster and underwent more identical development. For greenling, τ0 were 341.1 ± 3.60 min at 5°C, 275.5 ± 4.53 min at 10°C, 189.7 ± 6.93 min at 15°C, 99.2 ± 8.27 min at 20°C and 34.2 ± 8.74 min at 25°C. There were strong, negative correlations between the τ0 and water temperatures at all temperatures studied ( Y = ?79.3 X + 425.3, R 2 = 0.9968, where Y is the mitotic interval and X is the temperature). |
| |
| Keywords: | chromosome manipulation greenling Hexagrammos otakii mitotic interval temperature-dependent |
| 本文献已被 SpringerLink 等数据库收录！ |
|
