| 动物油脂中DNA的提取及牛、羊源性成分的PCR检测 |
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| 引用本文： | 邵碧英,张体银,李志方,郑腾,陈文炳. 动物油脂中DNA的提取及牛、羊源性成分的PCR检测[J]. 中国兽医学报, 2006, 26(3): 300-302 |
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| 作者姓名： | 邵碧英 张体银 李志方 郑腾 陈文炳 |
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| 作者单位： | 福建出入境检验检疫局,福建,福州,350003 |
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| 基金项目： | 国家质检总局资助项目;福建质检局科研项目 |
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| 摘 要： | 用异硫氰酸胍法提取鱼油、加入牛肉DNA的鱼油、加入山羊肉DNA的互油和牛油中的DNA。18S rDNA片段以及牛、羊源性成分的PCR扩增结果表明，建立的动物油脂DNA提取方法是可行的。用建立的DNA提取方法提取3份送检鱼油的DNA，牛、羊源性成分的PCR检测结果均为阴性。
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| 关 键 词： | 动物油脂 DNA提取 牛、羊源性成分 |
| 文章编号： | 1005-4545(2006)03-0300-03 |
| 收稿时间： | 2004-12-03 |
| 修稿时间： | 2004-12-03 |
The DNA Extraction and Detection of Bovine,Sheep and Goat Derived Material with PCR in Animal Oil |
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| SHAO Bi-ying,ZHANG Ti-yin,LI Zhi-fang,ZHENG Teng,CHEN Wen-bing. The DNA Extraction and Detection of Bovine,Sheep and Goat Derived Material with PCR in Animal Oil[J]. Chinese Journal of Veterinary Science, 2006, 26(3): 300-302 |
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| Authors: | SHAO Bi-ying ZHANG Ti-yin LI Zhi-fang ZHENG Teng CHEN Wen-bing |
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| Affiliation: | Fujian Entry-Exit Inspection and Quarantine Bureau ,Fuzhou 350003 ,China |
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| Abstract: | The total DNAs of fish oil,fish oil added with bovine DNA, fish oil added with goat DNA and butter were extracted by guanidine isothiocyanate method. The detection results of 18S ribosomal DNA fragment and bovine,sheep and goat derived material showed that the DNA extraction method for animal oil was practical. The total DNAs of three sifh oil samples supplied by applicant were extracted by the DNA extraction method,and the PCR detection results of bovine,sheep and goat derived material were all negative. |
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| Keywords: | PCR |
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