| 苹果汁中棒曲霉素主要产生菌的ITS序列分析鉴定 |
| |
| 引用本文： | 苏青峰,岳田利,袁亚红. 苹果汁中棒曲霉素主要产生菌的ITS序列分析鉴定[J]. 西北农林科技大学学报(自然科学版), 2005, 33(7): 131-134 |
| |
| 作者姓名： | 苏青峰 岳田利 袁亚红 |
| |
| 作者单位： | 西北农林科技大学,食品科学与工程学院,陕西,杨凌,712100 |
| |
| 基金项目： | 国家“十五”科技攻关项目(2001BA51A15)，科技部西部专项(2001BA901A19) |
| |
| 摘 要： | 从浓缩苹果汁生产原料中分离出多种棒曲霉素产生菌,形态学初步鉴定表明其均为青霉属和曲霉属真菌。为确定其分类地位,又用ITS-DNA测序鉴定进行了验证。将ITS-rDNA提取后进行PCR扩增,测序后提交到EuropeanMolecularBiologyLaboratory(EMBL)核酸数据库,通过Blast程序,将ITS-rDNA序列与Genebank中的核酸序列进行对照,结果确定其确为青霉属与曲霉属菌株。
|
| 关 键 词： | 棒曲霉素 扩张青霉 ITS-rDNA PCR 浓缩苹果汁 |
| 文章编号： | 1671-9387(2005)07-0131-04 |
| 收稿时间： | 2005-03-29 |
| 修稿时间： | 2005-03-29 |
Identification of dominating patulin-producing strain by ITS-DNA sequence analysis in apple juice concentrates |
| |
| SU Qing-feng,YUE Tian-li,YUAN Ya-hong,GAO Zhen-peng. Identification of dominating patulin-producing strain by ITS-DNA sequence analysis in apple juice concentrates[J]. Journal of Northwest A&F University（Natural Science Edition）, 2005, 33(7): 131-134 |
| |
| Authors: | SU Qing-feng YUE Tian-li YUAN Ya-hong GAO Zhen-peng |
| |
| Affiliation: | (College of Food Science and Engineering,Northwest A & F University,Yangling,Shaanxi 712100,China) |
| |
| Abstract: | Several patulin-producing strains isolated from raw material of apple juice concentrates were identificated in morphology,in which all of them were penicillium and aspergillus.Then ITS-rDNA sequence analysis was used to confirm it.The ITS-rDNA sequence was submitted to european molecular biology laboratory (EMBL),and contrasted with DNA sequence in genebank and confirmed that they were penicillium and aspergillus. |
| |
| Keywords: | patulin Penicillium expansum ITS-rDNA PCR apple juice concentrate |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《西北农林科技大学学报(自然科学版)》浏览原始摘要信息 |
|
点击此处可从《西北农林科技大学学报(自然科学版)》下载全文 |
