河套蜜瓜子叶诱导形成体细胞胚的研究

 以河套蜜瓜成熟种子子叶为外植体, 研究体细胞胚途径再生培养的主要影响因子, 并对体胚发生过程进行了观察。结果表明; 体细胞胚发生对培养基糖源浓度要求较高, MS附加60 g/L的糖源效果最好; 最佳植物生长调节剂组合为2,4-D 2.0 mg/L + 6-BA 1.0 mg/L; 1日苗龄子叶为最佳外植体源; 在诱导培养基上经暗培养1周、光培养1周, 再转入胚发育培养基, 有利于成熟胚的产生; 体细胞胚可产生在愈伤组织内部或表面、外植体表面、不定根上及外植体维管束的束中分生组织等部位。

Studies of Somatic Embryo via Cotyledon Induction in Cucumis melon L.‘Hetao’

The main factors of somatic embryo on Cucumis melon L.‘Hetao’from cotyledon explants were studied and embryogenesis was observed. The results indicate that the highest regeneration was occurred on MS medium containing 60 g/L sugar and supp lemented with 2.0 mg/L 2,4-D and 1.0 mg/L 6-BA. Daily cotyledon explants were cultured one week in darkness, followed by another week photoperiod before transfering to embryos development medium, produced more mature embryos. Somatic embryos were formed from the inside or the surface of callus, the surface of explant, adventitious root and meristem of explant vascular.