食品添加剂Na2 SO3对小鼠嗜多染红细胞微核率的影响

目的]了解经口进入机体的亚硫酸盐对哺乳动物的毒性效应。方法]以92只7～12周龄的健康昆明种小鼠为试材， 采用灌胃染毒法进行剂量效应关系和时间效应关系的微核试验。结果] Na2SO3能诱发小鼠骨髓嗜多染红细胞微核，且随剂量增高微核率明显增加（P＜0.01）。时间效应关系的微核试验表明，Na2SO3染毒后不同时间处死动物并制片，骨髓嗜多染红细胞微核率不同，染毒后12 h的微核率与阴性对照组相比有极显著差异（P＜0.01），随染毒后时间的延长，细胞微核率增加，24 h达到高峰，与相邻各组差异极显著（P＜0.01），之后随时间延长而降低，表明Na2SO3诱发小鼠骨髓嗜多染红细胞微核有一定时间效应关系。结论] 亚硫酸盐是哺乳动物染色体断裂剂和基因毒性因子。

Effect of Food Additive Na2 SO3 on the Micronucleus Rates of Mice Polychromatic Erythrocytes

Objective] The aim was to know about the toxic effect of Na2SO3 that entered into body via mouth on mammalian.Method] With 92 healthy Kunming mice of 7-12-week age as tested material,the micronucleus tests of dose-response relationship and time-effect relationship were performed by using stomach exposure method.Result] Na2SO3 could induce the micronucleus in polychromatic erythrocytes(PCE) from mice bone marrow,and the micronucleus rate was increased significantly with dose rising(P<0.01).The micronucleus test of time-effect relationship showed that animals were sacrificed in different time and the slice production after Na2SO3 exposure,the micronucleus rate of bone marrow PCE was varied,the micronucleus rate after 12h exposure had extremely significant difference compared with negative control group(P<0.01),along with the time extending after exposure,the micronucleus rate was increased and reached to the peak at 24h,having very significant difference with adjacent each group(P<0.01),and then was decreased with time prolonging,which indicated PCE micronucleus of mice bone marrow induced by Na2SO3 had some time-effect relationship.Conclusion] Sulfite was clastogen and gene virulence factor of mammals.