| 基于改良SDS法的杏基因组DNA提取 |
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| 引用本文： | 杨 丽,张俊环,孙浩元,王玉柱. 基于改良SDS法的杏基因组DNA提取[J]. 中国农学通报, 2008, 24(4): 69-71 |
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| 作者姓名： | 杨 丽 张俊环 孙浩元 王玉柱 |
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| 作者单位： | 北京市农林科学院林业果树研究所,北京,100093 |
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| 摘 要： | 为探讨改良SDS法是否适用于杏基因组DNA提取及提取液适宜浓度，以6个品种杏叶片为试材提取DNA，提取液设0.5%、1%、2%三个浓度梯度。检测结果表明，改良SDS法完全适用于杏叶片基因组DNA的提取，3种浓度的提取液均可得到质量较高的DNA， 且0.5%为改良SDS法提取杏叶片基因组DNA的适宜提取液浓度。
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| 关 键 词： | 白魔芋 白魔芋 种质资源 遗传分析 RAPD |
| 收稿时间： | 2008-02-29 |
| 修稿时间： | 2008-02-29 |
Study on Apricot Genomic DNA Extraction by Modified SDS Method |
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| Yang Li,Zhang Junhuan,Sun Haoyuan,Wang Yuzhu. Study on Apricot Genomic DNA Extraction by Modified SDS Method[J]. Chinese Agricultural Science Bulletin, 2008, 24(4): 69-71 |
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| Authors: | Yang Li Zhang Junhuan Sun Haoyuan Wang Yuzhu |
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| Affiliation: | Institute of Forestry & Pomology, Beijing Academy of Agriculture & Forestry Sciences, Beijing 100093 |
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| Abstract: | To probe the adaptability of modified SDS method in apricot genomic DNA extraction and the optimal concentration, the genomic DNA samples of 6 apricot cultivars were isolated by 0.5%, 1%, 2%SDS extracted buffer. The results showed that good quality DNA would be obtained by modified SDS method and the 0.5% is better. |
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| Keywords: | apricotzz DNA extractionzz SDS methodzz |
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