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| 利用AFLP分子标记对广西荔枝优稀种质遗传多样性及分类研究 | |
| 引用本文： | 彭宏祥,曹辉庆,朱建华,廖惠红,黄凤珠,李江舟,梁文. 利用AFLP分子标记对广西荔枝优稀种质遗传多样性及分类研究[J]. 西南农业学报, 2006, 19(1): 108-111 |
| 作者姓名： | 彭宏祥 曹辉庆 朱建华 廖惠红 黄凤珠 李江舟 梁文 |
| 作者单位： | 1. 广西农科院园艺研究所,广西,南宁,530007 2. 广西农科院园艺研究所,广西,南宁,530007;广西大学农学院,广西,南宁,530005 3. 广西大学农学院,广西,南宁,530005 |
| 基金项目： | 国家自然科学基金（30260056）;广西科学基金（桂科基0144005、0575033）;广西农科院科技发展基金（2002013）项目资助 |
| 摘 要： | 选用7对引物组合EcoR I AAG Mse I CAC、EcoR I AAC Mse I CAA等对27份荔枝材料进行多样性分析,共得到扩增位点392个,平均每对引物组合扩增位点56,多态性比例达100%,区分率100%。通过UPGMA进行聚类分析,根据相似系数0.61的水平可将27份荔枝种质材料(品种)分为7组,供试品种多态性比例介于19.51%~45.12%间,这一结论与形态学分类基本相符。 |
| 关 键 词： | 荔枝 AFLP 种质 遗传多样性 |
| 文章编号： | 1001-4829（2006）01-0108-04 |
| 收稿时间： | 2005-12-19 |
| 修稿时间： | 2005-12-19 |
Studies on the application of AFLP molecular markers on genetic diversity and classification of good and rare litchi resources in Guangxi |
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| PENG Hong-xiang,CAO Hui-qing,ZHU Jiang-hua,LIAO Hui-hong,HUANG Feng-zhu,LI Jiang-zhou,LIANG Wen. Studies on the application of AFLP molecular markers on genetic diversity and classification of good and rare litchi resources in Guangxi[J]. Southwest China Journal of Agricultural Sciences, 2006, 19(1): 108-111 | |
| Authors: | PENG Hong-xiang CAO Hui-qing ZHU Jiang-hua LIAO Hui-hong HUANG Feng-zhu LI Jiang-zhou LIANG Wen |
| Affiliation: | 1 .Horticulture Research Institute of Guangxi Academy of Agricultural Sciences, Guangxi Nanning 530007, China; 2.Agricultural College, Guangxi University, Guangxi Nanning 530005, China |
| Abstract: | Genetic diversity among 27 litchi varieties were studied using seven pairs of primer combinations such as EcoR I AAG Mse I CAC,EcoR I AAC Mse I CAA AFLP analysis.A total of 392 DNA fragments were scored among all materials,averaging 56 for each primer combination,of which,392(100 %) bands were polymorphic,100 % dividing rate with the cluster analysis of using unweighted Pair Group Method(UPGMA) and 0.61 similar coefficient showed that the 27 litchi varieties could be classified into seven distinct clusters,polymorphic proportion was 19.51 %-45.12 %,the conclusion was assimilate to morphological classifying. |
| Keywords: | litchi AFLP germplasm genetic diversity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |