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培养基酸度对皂荚茎段试管培养生长的影响
引用本文:兰彦平,顾万春,周连第,李淑英,曹庆昌.培养基酸度对皂荚茎段试管培养生长的影响[J].中国农学通报,2005,21(6):89-89.
作者姓名:兰彦平  顾万春  周连第  李淑英  曹庆昌
作者单位:1. 北京市农林科学院农业综合发展研究所,北京,100089
2. 中国林业科学研究院林业研究所,北京,100091
基金项目:"北京奥运绿化和美化科技工程-城市绿化关键技术研究"(2003BA904B07)及"十五"国家攻关"林木种质资源保存技术创新与利用研究"(2001BA511B10).
摘    要:研究了培养基酸度对皂荚茎段试管苗生长的影响,结果表明,在初始pH值为3.5或5.8的MS培养基中,皂荚茎段培养一定时间后,培养基pH值均有所下降,降低的幅度与接种的试管苗数目有关。初始pH值不同(3.5或5.8)的MS培养基中茎段的分化率间存在差异。

关 键 词:皂荚  pH值  组织培养

Effect of Medium Acidity on Growth of Gleditsia sinensis Growing in Vitro
Lan Yanping,Gu Wanchun,Zhou Liandi,Li Shuying,Cao Qingchang.Effect of Medium Acidity on Growth of Gleditsia sinensis Growing in Vitro[J].Chinese Agricultural Science Bulletin,2005,21(6):89-89.
Authors:Lan Yanping  Gu Wanchun  Zhou Liandi  Li Shuying  Cao Qingchang
Institution:(1Institute of Agriculture System and Development, Beijing Academy of Agriculture and Forestry, Beijing 100089; 2Research Institute of Forestry, China Academy of Forestry, Beijing 100091)
Abstract:Micropropagated Gleditsia sinensis was found to decrease the pH of Murashige and Skoog's plant tissue culture medium (initial pH 3.5 or 5.8) to different values depending on the numbers of plants per tub. The difference was found in the rate of differentiation after plants had been grown on medium initially adjusted or buffered to values 3.5 or 5.8.
Keywords:Gleditsia sinensis  pH value  Tissue culture
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