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| 玉米磷酸丙糖转移蛋白（TPT）基因全长cDNA克隆及其在烟草中表达 | |
| 引用本文： | 薛春生,肖淑芹,王国英,陈捷. 玉米磷酸丙糖转移蛋白（TPT）基因全长cDNA克隆及其在烟草中表达[J]. 农业生物技术学报, 2008, 16(6) |
| 作者姓名： | 薛春生 肖淑芹 王国英 陈捷 |
| 作者单位： | 1. 沈阳农业大学分子与生理植物病理学研究室,沈阳,110161;中国农业大学农业生物技术国家重点实验室,北京,100094 2. 中国农业大学农业生物技术国家重点实验室,北京,100094 3. 上海交通大学农业与生物学院,上海,201101 |
| 基金项目： | 国家转基因植物专项基金 |
| 摘 要： | 以玉米黄早四幼苗叶片为材料，通过RT-PCR扩增后获得玉米磷酸丙糖转移蛋白(Triose phosphate translocator．TPT)cDNA 全长片段，对其进行序列分析。结果表明：分离的目的片段核苷酸序列与报道的相比同源率为99.9％，只有1个碱基发生改变。将得到的玉米 cDNA与CaMV35S启动子和NOS终止子融合，构建了正义表达载体，通过根癌土壤杆菌介导转化烟草，转基因植株经PCR和Southern杂交检测，证明TPT基因已整合到烟草基因组上 |
| 关 键 词： | 玉米磷酸丙糖转移蛋白 cDNA克隆 烟草 表达 |
| 收稿时间： | 2007-06-18 |
| 修稿时间： | 2008-06-03 |
Cloning of Full Length cDNA of Triose Phosphate Translocator from Corn and Its Expression in Tobacco |
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| XUE Chun-sheng,XIAO Shu-qin,WANG Guo-ying,CHEN Jie. Cloning of Full Length cDNA of Triose Phosphate Translocator from Corn and Its Expression in Tobacco[J]. Journal of Agricultural Biotechnology, 2008, 16(6) | |
| Authors: | XUE Chun-sheng XIAO Shu-qin WANG Guo-ying CHEN Jie |
| Abstract: | The triose phosphate translocator(TPT)cDNA of full length was amplified by RT-PCR. Sequence analysis indicated that the full length cDNA fragment was showing 99.9％ homology to the sequence reported before．Only one base is different．The sense expression vector with TPT full length cDNA underthe control of CaMV 35S promoter was constructed and were transformed into tobacco by leaf disc transformation．The transformed tobaccos were identified by PCR and Southern blotting analysis． |
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