| 百脉根BIO和豌豆突变位点ELE2的比较基因组定位 |
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| 引用本文： | 李信,赫圣博,庄黎丽,Sato Shusei,Tabata Satoshi,Ambrose Mike,Rameau Catherine,杨军,胡筱荷,罗达.百脉根BIO和豌豆突变位点ELE2的比较基因组定位[J].广西农业生物科学,2010(1):3-9. |
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| 作者姓名： | 李信 赫圣博 庄黎丽 Sato Shusei Tabata Satoshi Ambrose Mike Rameau Catherine 杨军 胡筱荷 罗达 |
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| 作者单位： | 上海交通大学生命科学技术学院;Kazusa;Research;Institute;2-6-7;Kazusa-kamatari;Depart-ment;Crop;Genetics;John;Innes;Centre;Colney;Norwich;Station;Génétique;d'Amélioration;Plantes;Institut;J.P.;Bourgin;UR254;INRA;中国科学院;上海生命科学研究院植物生理生态研究所植物分子遗传学国家重点实验室; |
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| 基金项目： | supported by projects (2008ZX08009-003;2009ZX08009-112B) from the ministry of agriculture of Chinafor transgenic research |
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| 摘 要： | 豆科两侧对称花的花瓣具有背腹（DV）的分化以及可变的器官内部（IN）非对称性,在大小与形状上显示出不同的发育特征;因而花瓣的发育为克隆决定植物器官的形状与大小的关键基因提供了很好的实验系统。本研究对百脉根中BIO基因进行研究。百脉根bio突变体具有多效性,既影响花器官内部的对称性也影响器官的大小和育性,豌豆ele突变体的表型与bio相似。定位结果表明BIO和ELE2位于豆科基因组的共线性区段,提示BIO和ELE2可能是同源基因突变所致。本研究利用比较基因组定位方法,将BIO和ELE2候选基因锚定在豆科模式植物百脉根和蒺藜苜蓿基因组含有11个同源基因的BAC重叠群上。BIO和ELE2基因的克隆将有助于揭示豆科花瓣形态和大小调控的分子机理,进而为豆科作物遗传改良提供分子理论基础。
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| 关 键 词： | 器官的形状和大小 百脉根 BIO基因 ELE2基因 比较基因组定位 |
Genetic Analysis and Comparative Mapping of BIO and ELE2 Loci in Lotus japonicus and Pea |
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| Li Xin,He Shengbo,Zhuang Lili,Sato Shusei,Tabata Satoshi,Ambrose Mike Rameau Catherine,Yang Jun,Hu Xiaohe,and Luo Da.Genetic Analysis and Comparative Mapping of BIO and ELE2 Loci in Lotus japonicus and Pea[J].Journal of Guangxi Agricultural and Biological Science,2010(1):3-9. |
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| Authors: | Li Xin He Shengbo Zhuang Lili Sato Shusei Tabata Satoshi Ambrose Mike Rameau Catherine Yang Jun Hu Xiaohe and Luo Da |
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| Institution: | Li Xin He Shengbo Zhuang Lili Sato Shusei Tabata Satoshi Ambrose Mike Rameau Catherine Yang Jun Hu Xiaohe and Luo Da (School of Life Science and Biotechnology,Shanghai Jiaotong University,Shanghai,200240; 2 Kazusa DNA Research Institute,2-6-7 Kazusa-kamatari,Kisarazu,Chiba,292-0818; 3 Department of Crop Genetics,John Innes Centre,Colney,Norwich,NR4 7UH; 4 Station de Génétique et d＇Amélioration des Plantes,Institut J.P. Bourgin,UR 254 INRA; 5 National Key Laboratory of Plant Molecular Genetics,Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai,200032) |
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| Abstract: | Petals in zygomorphic flowers possess dorsoventral (DV) differentiation and variable organ internal (IN) asymmetry,which can be clearly defined by their shape and size,and thus provides a good experimental sys-tem to identify the key regulators in the control of organ shape and size. In this study,a novel locus,BIO ORGANS (BIO) was investigated in Lotus japonicus,whose mutants display deficiency of IN asymmetry in flowers,as well as the pleiotropic defects of enlarged organs and infertility which are simila... |
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| Keywords: | Organ shape and size L japonicus Pea BIO ELE2 Comparative mapping |
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