维拉帕米和三苯氧胺逆转卵巢癌细胞对阿霉素耐药性的实验研究

目的 了解维拉帕米和三苯氧胺体外逆转卵巢癌细胞阿霉素耐药性的效果及临床意义.方法 分别以阿霉素、维拉帕米和三苯氧胺作用于人卵巢癌亲本细胞SKOV3和阿霉素耐药细胞SKOV3/ADM后,采用MTI法检测药物对癌细胞的抑制作用,以细胞内阿霉素聚集量测定和流式细胞术检测凋亡.结果 维拉帕米和三苯氧胺能部分逆转耐药细胞对阿霉素的耐药性,且维拉帕米能显著增加耐药细胞内阿霉素的聚集量并促进其凋亡.结论 虽然维拉帕米和三苯氧胺能部分逆转耐药细胞对阿霉素的耐药性,但用于临床的意义不大.

Reversal of adraimycin-resistance in ovarian carcinoma cells by verapamil and tamoxifen in vitro

Objective To investigate the reversal effect and clinical significance of verapamil and tamoxifen on adraimycin-resistance ovarian carcinoma cell line in vitro. Methods SKOV3 and SKOV3/ADM cell lines were treated with adraimycin, verapamil and tamoxifen, and then the cell inhibition, intracellular adraimycin accumulation and apoptosis were detected by MTT, spectrofluorimetry and flow cytometry, respectively. Results Adraimycin-resistance in ovarian carcinoma cells was partly reversed by verapamil and tamoxifen. Verapamil increased the intracellular adraimycin concentration and enhanced the apoptosis in SKOV3/ADM cells. Conclusion Verapamil and tamoxifen could partly reverse adraimycin-resistance in ovarian carcinoma cells in vitro, but they have little clinical value.