| 水磨年糕中微生物的分离纯化与鉴定 |
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| 引用本文： | 陈挺,娄永江,庞林江,王茜茜. 水磨年糕中微生物的分离纯化与鉴定[J]. 安徽农学通报, 2015, 0(19): 25-28. DOI: 10.3969/j.issn.1007-7731.2015.19.010 |
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| 作者姓名： | 陈挺 娄永江 庞林江 王茜茜 |
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| 作者单位： | 1. 宁波大学海洋学院，浙江宁波 315211; 宁波市江北区科技局，浙江宁波 315211;2. 宁波大学海洋学院,浙江宁波,315211;3. 浙江农林大学农业与食品科学学院,浙江杭州,311300 |
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| 基金项目： | 国家星火计划项目，宁波市科技计划项目 |
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| 摘 要： | 通过对年糕内部和表面的微生物进行分离纯化,并通过形态观察、生化测定的方法来鉴定微生物的组成,结果表明:年糕表面的微生物主要有6种细菌、3种霉菌和2种酵母菌。细菌中4种是芽孢杆菌,分别为巨大芽孢杆菌(Bacillus megaterium)、枯草芽孢杆菌(Bacillus subtilis)、蜡状芽孢杆菌(Bacillus cereus)和短芽孢杆菌(Bacillus brevis),2种是非芽孢杆菌,分别为乙酰短杆菌(Brevibacterium acetylicum)和乙酸钙不动杆菌(Acinetobacter calcoaceticus);霉菌分别为米根霉(Rhizopus oryzae)、构巢曲霉(Aspergillus nidulans)和米曲霉(Aspergillus oryzae);酵母菌分别为茁芽丝孢酵母(Trichosporon pullulans)和白色假丝酵母(Candida albicans)。年糕内部的微生物有巨大芽孢杆菌、枯草芽孢杆菌、短芽孢杆菌和白色假丝酵母,无霉菌。
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| 关 键 词： | 年糕 分离纯化 微生物 鉴定 |
Purification and Identification of Microbes in Water Mill Rice Cake |
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| Abstract: | Through the morphology observation and the physiological and biochemical of microorganisms which were separated and purified from the interior and the surface of the rice cake,we can get that there were 6 kinds of bacteri?um(involving 4 kinds of Bacillus which were Bacillus megaterium,Bacillus subtilis,Bacillus cereus and Bacillus bre?vis,2 kinds of non-Bacillus were Brevibacterium acetylicum and Acinetobacter calcoaceticus),3 kinds of molds(Rhizo?pus oryzae,Aspergillus oryzae and Aspergillus nidulans),and 2 kinds of Yeast(they were respectively Candida albi?cans and Trichosporon pullulans)on the surface of rice cake.The inside microorganisms of Rice cake were Bacillus megaterium,Bacillus subtilis,Bacillus brevis and trichosporon pullulans without mold. |
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| Keywords: | Rice cakes Separation and purification Microbes Identification |
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