流动注射-共振光散射联用技术测定硫氰酸根含量的研究

目的]探讨测定蔬菜硫氰酸根(SCN-)含量的新方法。方法]在醋酸缓冲介质及SCN-存在条件下,抗坏血酸将Cu(Ⅱ)还原为Cu(Ⅰ),Cu(Ⅰ)进一步与SCN-形成CuSCN沉淀,利用这种沉淀反应,结合流动注射技术,建立了测定SCN-的共振光散射新方法。结果]该方法线性范围为3~160μg/ml的SCN-,检出限达0.167μg/ml的SCN-。结论]拟定的分析方法简便、试剂少、线性范围宽,可用于蔬菜中SCN-含量的测定。

Study on the Determination of Thiocyanate Content through Couplining Technique of Flow Injection Analysis with Resonance Light Scattering

Objective] The aim was to discuss the new method of determining thiocyanate(SCN-) content in vegetables.Method] CuSCN,which Cu(Ⅱ) could be reduced to Cu(Ⅰ) by ascorbic acid in the presence of acetic acid buffer medium and SCN-,then Cu(Ⅰ) was precipitated with SCN-.Using the precipitation reaction and combined with flowinjection method,the new method of flow-injection resonance light scattering was established to determine SCN-.Result]The linear range of determination was 3-160 μg/ml.The detection limit was 0.167 g /ml SCN-.Conclusion] The confirmed method were simple,a little reagent and wide linear range.The method was could be applied to the determination of thiocyanate in vegetables.